Recent Advancements in the Production of Rhamnolipid Biosurfactants by

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 May 6

PMID 35519061

Authors

Parisa Eslami

Hamidreza Hajfarajollah

Shayesteh Bazsefidpar

Affiliations

Soon will be listed here.

Abstract

Rhamnolipid (RL) biosurfactant which is produced by species is one of the most effective surface-active agents investigated in the literature. Over the years, many efforts have been made and an array of techniques has been developed for the isolation of RL produced strains as well as RL homolog characterization. Reports show that RL productivity by the best-known producer, , is very diverse, from less than 1 gr/l to more than 200 g L. There are some major parameters that can affect RL productivity. These are culture conditions, medium composition, the mode of operation (batch, fed-batch and continuous), bioengineering/gene manipulation and finally extraction methods. The present paper seeks to provide a comprehensive overview on the production of rhamnolipid biosurfactant by different species of bacteria. In addition, we have extensively reviewed their potential for possible future applications.

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References

Chayabutra C, Wu J, Ju L . Rhamnolipid production by Pseudomonas aeruginosa under denitrification: effects of limiting nutrients and carbon substrates. Biotechnol Bioeng. 2000; 72(1):25-33. DOI: 10.1002/1097-0290(20010105)72:1<25::aid-bit4>3.0.co;2-j. View

Nguyen T, Edelen A, Neighbors B, Sabatini D . Biocompatible lecithin-based microemulsions with rhamnolipid and sophorolipid biosurfactants: formulation and potential applications. J Colloid Interface Sci. 2010; 348(2):498-504. DOI: 10.1016/j.jcis.2010.04.053. View

Zhao F, Jiang H, Sun H, Liu C, Han S, Zhang Y . Production of rhamnolipids with different proportions of mono-rhamnolipids using crude glycerol and a comparison of their application potential for oil recovery from oily sludge. RSC Adv. 2022; 9(6):2885-2891. PMC: 9059948. DOI: 10.1039/c8ra09351b. View

Kim S, Kim Y, Lee S, Kim J, Yun M, Kim I . Insecticidal activity of rhamnolipid isolated from pseudomonas sp. EP-3 against green peach aphid (Myzus persicae). J Agric Food Chem. 2011; 59(3):934-8. DOI: 10.1021/jf104027x. View

Goswami D, Handique P, Deka S . Rhamnolipid biosurfactant against Fusarium sacchari--the causal organism of pokkah boeng disease of sugarcane. J Basic Microbiol. 2013; 54(6):548-57. DOI: 10.1002/jobm.201200801. View

Gudina E, Rodrigues A, de Freitas V, Azevedo Z, Teixeira J, Rodrigues L . Valorization of agro-industrial wastes towards the production of rhamnolipids. Bioresour Technol. 2016; 212:144-150. DOI: 10.1016/j.biortech.2016.04.027. View

Diaz De Rienzo M, Stevenson P, Marchant R, Banat I . Effect of biosurfactants on Pseudomonas aeruginosa and Staphylococcus aureus biofilms in a BioFlux channel. Appl Microbiol Biotechnol. 2016; 100(13):5773-9. PMC: 4909806. DOI: 10.1007/s00253-016-7310-5. View

Tavares L, Silva P, Junqueira M, Mariano D, Nogueira F, Domont G . Characterization of rhamnolipids produced by wild-type and engineered Burkholderia kururiensis. Appl Microbiol Biotechnol. 2012; 97(5):1909-21. DOI: 10.1007/s00253-012-4454-9. View

Van Hamme J, Singh A, Ward O . Physiological aspects. Part 1 in a series of papers devoted to surfactants in microbiology and biotechnology. Biotechnol Adv. 2006; 24(6):604-20. DOI: 10.1016/j.biotechadv.2006.08.001. View

10.

Kuyukina M, Ivshina I, Gein S, Baeva T, Chereshnev V . In vitro immunomodulating activity of biosurfactant glycolipid complex from Rhodococcus ruber. Bull Exp Biol Med. 2008; 144(3):326-30. DOI: 10.1007/s10517-007-0324-3. View

11.

Davis D, Lynch H, Varley J . The application of foaming for the recovery of Surfactin from B. subtilis ATCC 21332 cultures. Enzyme Microb Technol. 2001; 28(4-5):346-354. DOI: 10.1016/s0141-0229(00)00327-6. View

12.

Campos J, Stamford T, Sarubbo L, de Luna J, Rufino R, Banat I . Microbial biosurfactants as additives for food industries. Biotechnol Prog. 2013; 29(5):1097-108. DOI: 10.1002/btpr.1796. View

13.

Deziel E, Lepine F, Dennie D, Boismenu D, Mamer O, Villemur R . Liquid chromatography/mass spectrometry analysis of mixtures of rhamnolipids produced by Pseudomonas aeruginosa strain 57RP grown on mannitol or naphthalene. Biochim Biophys Acta. 1999; 1440(2-3):244-52. DOI: 10.1016/s1388-1981(99)00129-8. View

14.

Partovi M, Bagheri Lotfabad T, Roostaazad R, Bahmaei M, Tayyebi S . Management of soybean oil refinery wastes through recycling them for producing biosurfactant using Pseudomonas aeruginosa MR01. World J Microbiol Biotechnol. 2013; 29(6):1039-47. DOI: 10.1007/s11274-013-1267-7. View

15.

Hayashi K . A rapid determination of sodium dodecyl sulfate with methylene blue. Anal Biochem. 1975; 67(2):503-6. DOI: 10.1016/0003-2697(75)90324-3. View

16.

Zhang L, Veres-Schalnat T, Somogyi A, Pemberton J, Maier R . Fatty acid cosubstrates provide β-oxidation precursors for rhamnolipid biosynthesis in Pseudomonas aeruginosa, as evidenced by isotope tracing and gene expression assays. Appl Environ Microbiol. 2012; 78(24):8611-22. PMC: 3502905. DOI: 10.1128/AEM.02111-12. View

17.

Zhang Y, Miller R . Enhanced octadecane dispersion and biodegradation by a Pseudomonas rhamnolipid surfactant (biosurfactant). Appl Environ Microbiol. 1992; 58(10):3276-82. PMC: 183091. DOI: 10.1128/aem.58.10.3276-3282.1992. View

18.

Witek-Krowiak A, Witek J, Gruszczynska A, Szafran R, Kozlecki T, Modelski S . Ultrafiltrative separation of rhamnolipid from culture medium. World J Microbiol Biotechnol. 2011; 27(8):1961-1964. PMC: 3140937. DOI: 10.1007/s11274-011-0655-0. View

19.

Zhao F, Mandlaa M, Hao J, Liang X, Shi R, Han S . Optimization of culture medium for anaerobic production of rhamnolipid by recombinant Pseudomonas stutzeri Rhl for microbial enhanced oil recovery. Lett Appl Microbiol. 2014; 59(2):231-7. DOI: 10.1111/lam.12269. View

20.

Beuker J, Steier A, Wittgens A, Rosenau F, Henkel M, Hausmann R . Integrated foam fractionation for heterologous rhamnolipid production with recombinant Pseudomonas putida in a bioreactor. AMB Express. 2016; 6(1):11. PMC: 4747948. DOI: 10.1186/s13568-016-0183-2. View