Colorimetric Detection of Hydrogen Peroxide and Glucose by Exploiting the Peroxidase-like Activity of Papain

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 May 6

PMID 35516354

Authors

Yuye Chen

Qingmei Zhong

Yilin Wang

Chunling Yuan

Xiu Qin

Yuanjin Xu

Affiliations

Soon will be listed here.

Abstract

Papain, a natural plant protease that exists in the latex of , catalyzes the hydrolysis of peptide, ester and amide bonds. In this work, we found that papain displayed peroxidase-like activity and catalyzed the oxidation of 3,3',5',5'-tetramethylbenzidine (TMB) in the presence of HO. This results in the formation of a blue colored product with an absorption maximum at 652 nm. The effects of experimental parameters including pH and reaction temperature on catalytic activity of papain were investigated. The increase of absorbance induced by the catalytic effect of papain offers accurate detection of HO in the range of 5.00-90.0 μM, along with a detection limit of 2.10 μM. A facile colorimetric method for glucose detection was also proposed by combining the glucose oxidase (GOx)-catalyzed glucose oxidation and papain-catalyzed TMB oxidation, which exhibited a linear response in the range of 0.05-0.50 mM with a detection limit of 0.025 mM. The method proposed here displayed excellent selectivity, indicating that common coexisting substances (urea, uric acid, ascorbic acid, maltose, lactose and fructose) in urine did not interfere with detection of glucose. More importantly, the suggested method was successfully used to precisely detect the glucose concentration in human urine samples with recoveries over 96.0%.

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