Phenylboronic Acid Derivative-modified (6,5) Single-wall Carbon Nanotube Probes for Detecting Glucose and Hydrogen Peroxide

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 May 6

PMID 35516097

Authors

Yunfan Qiao

Rushi Zhao

Min Zhang

Hongyang Zhang

Yuerong Wang

Ping Hu

Affiliations

Soon will be listed here.

Abstract

In this paper, we presented a new method for constructing near-infrared fluorescence probes and their applications in detecting glucose and hydrogen peroxide (HO). We used purified (6,5) single-wall carbon nanotubes (SWCNTs) separated by a polyethylene glycol/dextran aqueous two-phase system as the basis for near-infrared probes. Different phenylboronic acids were used for non-covalent modification of SWCNTs (6,5). Glucose was detected by the specific binding of the boronic acid group with -diol. Hydrogen peroxide was detected by horseradish peroxidase (HRP) combined with phenylboronic-acid-modified SWCNTs. The results revealed that the fluorescence intensity of purified SWCNTs was significantly enhanced without other chiral nanotube interactions compared to that of the raw SWCNT material. The fluorescence responses of 3-carboxy-5-nitrophenylboronic acid-modified purified CNTs could be used to effectively measure glucose in the concentration range from 0.01 mM to 0.50 mM with an interval linear index of = 0.996 (LOD = 1.7 μM, S/N = 3). The detected HO with the 3-aminobenzeneboronic acid-modified (6,5) SWCNT with HRP was in the concentration range from 5.0 μM to 40 μM with an interval linear index of = 0.997 (LOD = 0.85 μM, S/N = 3). Moreover, this sensor exhibited a strong anti-interference effect without biological matrix fluorescence effects above 1000 nm wavelength. Thus, the proposed novel near-infrared fluorescence probes employed for reducing the interference of the biomatrix in this region are expected to be used in subsequent biosensor investigations.

Citing Articles

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