Natural Heterogeneous Catalysis with Immobilised Oxidase Biocatalysts

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 May 6

PMID 35515476

Authors

Ashley P Mattey

Jack J Sangster

Jeremy I Ramsden

Christopher Baldwin

William R Birmingham

Rachel S Heath

Antonio Angelastro

Nicholas J Turner

Sebastian C Cosgrove

Sabine L Flitsch

Affiliations

Soon will be listed here.

Abstract

The generation of immobilised oxidase biocatalysts allowing multifunctional oxidation of valuable chemicals using molecular oxygen is described. Engineered galactose oxidase (GOase) variants M and M, an engineered choline oxidase (AcCO6) and monoamine oxidase (MAO-N D9) displayed long-term stability and reusability over several weeks when covalently attached on a solid support, outperforming their free counterparts in terms of stability (more than 20 fold), resistance to heat at 60 °C, and tolerance to neat organic solvents such as hexane and toluene. These robust heterogenous oxidation catalysts can be recovered after each reaction and be reused multiple times for the oxidation of different substrates.

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