Highly Rapid and Non-enzymatic Detection of Cholesterol Based on Carbon Nitride Quantum Dots As Fluorescent Nanoprobes

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 May 6

PMID 35515374

Authors

Ying Chen

Gege Yang

Shanshan Gao

Liangliang Zhang

Mengdi Yu

Chunxia Song

Ying Lu

Affiliations

Soon will be listed here.

Abstract

In this work, we reported a highly rapid and non-enzymatic method for cholesterol measuring based on carbon nitride quantum dots (CNQDs) as fluorescent nanoprobes, which were synthesized through chemical oxidation. The obtained CNQDs displayed high quantum yield up to 35% as well as excellent photostability, water solubility and low toxicity. We found that the fluorescence of CNQDs could be quenched more than 90% within 30 seconds by cholesterol through the formation of hydrogen bonds between -NH, -NH on the surface of CNQDs and cholesterol containing -OH. According to this phenomenon, a cholesterol detection method was constructed with a wide linear region over the range of 0-500 μmol L and a detection limit as low as 10.93 μmol L, and it possessed the obvious advantages of being a very rapid process and avoiding the use of enzymes. In addition, this method showed high selectivity in the presence of various interfering reagents and applicability to the measurement of cholesterol in fetal bovine serum, which indicated its potential application value in clinical settings.

Citing Articles

Platinum Nanoparticles Loaded Graphitic Carbon Nitride Nanosheets with Enhanced Peroxidase-like Activity for HO and Oxidase-Based Sensing.

Yang G, Chen Y, Shi R, Chen R, Gao S, Zhang X Molecules. 2023; 28(9).

PMID: 37175146 PMC: 10179752. DOI: 10.3390/molecules28093736.

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