Primary Structure of Human Neutrophil Elastase

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1987 Apr 1

PMID 3550808

Citations 60

Authors

S Sinha

W Watorek

S Karr

J Giles

W Bode

J Travis

Affiliations

Soon will be listed here.

Abstract

The complete amino acid sequence of human neutrophil elastase has been determined. The protein consists of 218 amino acid residues, contains two asparagine-linked carbohydrate side chains, and is joined together by four disulfide bonds. Comparison of the sequence to other serine proteinases indicates only moderate homology with porcine pancreatic elastase (43.0%) or neutrophil cathepsin G (37.2%). In particular, many of the residues suggested to play important roles in the mechanism by which the pancreatic elastase functions are significantly changed in the neutrophil enzyme, indicating alternative types of binding with the human proteinase.

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References

RAFTERY M, Cole R . On the aminoethylation of proteins. J Biol Chem. 1966; 241(15):3457-61. View

Crestfield A, Moore S, STEIN W . The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins. J Biol Chem. 1963; 238:622-7. View

Janoff A, Scherer J . Mediators of inflammation in leukocyte lysosomes. IX. Elastinolytic activity in granules of human polymorphonuclear leukocytes. J Exp Med. 1968; 128(5):1137-55. PMC: 2138566. DOI: 10.1084/jem.128.5.1137. View

SHOTTON D, Hartley B . Amino-acid sequence of porcine pancreatic elastase and its homologies with other serine proteinases. Nature. 1970; 225(5235):802-6. DOI: 10.1038/225802a0. View

SHOTTON D, White N, Watson H . Conformational changes and inhibitor binding at the active site of elastase. Cold Spring Harb Symp Quant Biol. 1972; 36:91-105. DOI: 10.1101/sqb.1972.036.01.015. View

Janoff A . Purification of human granulocyte elastase by affinity chromatography. Lab Invest. 1973; 29(4):458-64. View

DEWALD B, Bretz U, Baggiolini M . Subcellular localization and heterogeneity of neutral proteases in neutrophilic polymorphonuclear leukocytes. J Exp Med. 1975; 141(4):709-23. PMC: 2189758. DOI: 10.1084/jem.141.4.709. View

Baugh R, Travis J . Human leukocyte granule elastase: rapid isolation and characterization. Biochemistry. 1976; 15(4):836-41. DOI: 10.1021/bi00649a017. View

Starkey P, Barrett A . Neutral proteinases of human spleen. Purification and criteria for homogeneity of elastase and cathepsin G. Biochem J. 1976; 155(2):255-63. PMC: 1172830. DOI: 10.1042/bj1550255. View

10.

Wyckoff M, Rodbard D, Chrambach A . Polyacrylamide gel electrophoresis in sodium dodecyl sulfate-containing buffers using multiphasic buffer systems: properties of the stack, valid Rf- measurement, and optimized procedure. Anal Biochem. 1977; 78(2):459-82. DOI: 10.1016/0003-2697(77)90107-5. View

11.

Blow A . Action of human lysosomal elastase on the oxidized B chain of insulin. Biochem J. 1977; 161(1):13-6. PMC: 1164467. DOI: 10.1042/bj1610013. View

12.

Ashe B, Zimmerman M . Specific inhibition of human granulocyte elastase by cis-unsaturated fatty acids and activation by the corresponding alcohols. Biochem Biophys Res Commun. 1977; 75(1):194-9. DOI: 10.1016/0006-291x(77)91308-0. View

13.

Twumasi D, Liener I, GALDSTON M, Levytska V . Activation of human leukocyte elastase by human alpha2-macroglobulin. Nature. 1977; 267(5606):61-3. DOI: 10.1038/267061a0. View

14.

Roughley P, Barrett A . The degradation of cartilage proteoglycans by tissue proteinases. Proteoglycan structure and its susceptibility to proteolysis. Biochem J. 1977; 167(3):629-37. PMC: 1183709. DOI: 10.1042/bj1670629. View

15.

Roughley P . The degradation of cartilage proteoglycans by tissue proteinases. Proteoglycan heterogeneity and the pathway of proteolytic degradation. Biochem J. 1977; 167(3):639-46. PMC: 1183710. DOI: 10.1042/bj1670639. View

16.

Mahoney W, Hermodson M . High-yield cleavage of tryptophanyl peptide bonds by o-iodosobenzoic acid. Biochemistry. 1979; 18(17):3810-4. DOI: 10.1021/bi00584a026. View

17.

Reilly C, Travis J . The degradation of human lung elastin by neutrophil proteinases. Biochim Biophys Acta. 1980; 621(1):147-57. DOI: 10.1016/0005-2795(80)90070-7. View

18.

Matheson N, Janoff A, Travis J . Enzymatic oxidation of alpha-1-proteinase inhibitor in abnormal tissue turnover. Mol Cell Biochem. 1982; 45(2):65-71. DOI: 10.1007/BF00223501. View

19.

Travis J, Salvesen G . Human plasma proteinase inhibitors. Annu Rev Biochem. 1983; 52:655-709. DOI: 10.1146/annurev.bi.52.070183.003255. View

20.

Jekel P, Weijer W, Beintema J . Use of endoproteinase Lys-C from Lysobacter enzymogenes in protein sequence analysis. Anal Biochem. 1983; 134(2):347-54. DOI: 10.1016/0003-2697(83)90308-1. View