Structure of a Cytotoxic T-lymphocyte-specific Gene Shows a Strong Homology to Fibrinogen Beta and Gamma Chains

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1987 Mar 1

PMID 3550794

Citations 32

Authors

T Koyama

L R HALL

W G Haser

S Tonegawa

H Saito

Affiliations

Soon will be listed here.

Abstract

Using a subtractive cDNA cloning method, we isolated a number of T-lymphocyte-specific genes. One of these genes, represented by the cDNA clone pT49, is expressed in cytotoxic T lymphocytes but not in helper T lymphocytes or B lymphocytes. The protein structure deduced from the nucleotide sequence showed a high degree of homology to fibrinogen beta and gamma subunits. This might indicate that evolutionarily fibrinogen has its origin in lymphocytes. In spite of the strong homology of pT49 protein to the fibrinogen subunits, the positions of the introns in the pT49 gene are totally different from those of the fibrinogen genes.

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References

Young J, COHN Z . Cell-mediated killing: a common mechanism?. Cell. 1986; 46(5):641-2. DOI: 10.1016/0092-8674(86)90336-3. View

Brunet J, Dosseto M, Denizot F, Mattei M, Clark W, Haqqi T . The inducible cytotoxic T-lymphocyte-associated gene transcript CTLA-1 sequence and gene localization to mouse chromosome 14. Nature. 1986; 322(6076):268-71. DOI: 10.1038/322268a0. View

Reid K . Immune system. Complement-like cytotoxicity?. Nature. 1986; 322(6081):684. DOI: 10.1038/322684a0. View

Pasternack M, Verret C, Liu M, Eisen H . Serine esterase in cytolytic T lymphocytes. Nature. 1986; 322(6081):740-3. DOI: 10.1038/322740a0. View

Tschopp J, Masson D, Stanley K . Structural/functional similarity between proteins involved in complement- and cytotoxic T-lymphocyte-mediated cytolysis. Nature. 1986; 322(6082):831-4. DOI: 10.1038/322831a0. View

Chou P, Fasman G . Prediction of protein conformation. Biochemistry. 1974; 13(2):222-45. DOI: 10.1021/bi00699a002. View

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

Benton W, Davis R . Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977; 196(4286):180-2. DOI: 10.1126/science.322279. View

Doolittle R, Goldbaum D, Doolittle L . Designation of sequences involved in the "coiled-coil" interdomainal connections in fibrinogen: constructions of an atomic scale model. J Mol Biol. 1978; 120(2):311-25. DOI: 10.1016/0022-2836(78)90070-0. View

10.

Watt K, Takagi T, Doolittle R . Amino acid sequence of the beta chain of human fibrinogen. Biochemistry. 1979; 18(1):68-76. DOI: 10.1021/bi00568a011. View

11.

Doolittle R, Watt K, Cottrell B, Strong D, Riley M . The amino acid sequence of the alpha-chain of human fibrinogen. Nature. 1979; 280(5722):464-8. DOI: 10.1038/280464a0. View

12.

Chang T, Eisen H . Effects of N alpha-tosyl-L-lysyl-chloromethylketone on the activity of cytotoxic T lymphocytes. J Immunol. 1980; 124(3):1028-33. View

13.

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

14.

Maki R, Traunecker A, Sakano H, Roeder W, Tonegawa S . Exon shuffling generates an immunoglobulin heavy chain gene. Proc Natl Acad Sci U S A. 1980; 77(4):2138-42. PMC: 348667. DOI: 10.1073/pnas.77.4.2138. View

15.

Hanahan D, Meselson M . Plasmid screening at high colony density. Gene. 1980; 10(1):63-7. DOI: 10.1016/0378-1119(80)90144-4. View

16.

Chung D, Rixon M, MacGillivray R, Davie E . Characterization of a cDNA clone coding for the beta chain of bovine fibrinogen. Proc Natl Acad Sci U S A. 1981; 78(3):1466-70. PMC: 319151. DOI: 10.1073/pnas.78.3.1466. View

17.

Mount S . A catalogue of splice junction sequences. Nucleic Acids Res. 1982; 10(2):459-72. PMC: 326150. DOI: 10.1093/nar/10.2.459. View

18.

Crabtree G, Kant J . Organization of the rat gamma-fibrinogen gene: alternative mRNA splice patterns produce the gamma A and gamma B (gamma ') chains of fibrinogen. Cell. 1982; 31(1):159-66. DOI: 10.1016/0092-8674(82)90415-9. View

19.

Doolittle R . The structure and evolution of vertebrate fibrinogen. Ann N Y Acad Sci. 1983; 408:13-27. DOI: 10.1111/j.1749-6632.1983.tb23231.x. View

20.

Chung D, Que B, Rixon M, Mace Jr M, Davie E . Characterization of complementary deoxyribonucleic acid and genomic deoxyribonucleic acid for the beta chain of human fibrinogen. Biochemistry. 1983; 22(13):3244-50. DOI: 10.1021/bi00282a032. View