Antioxidant Activity of SSeCAHK in HepG2 Cells: a Selenopeptide Identified from Selenium-enriched Soybean Protein Hydrolysates

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 May 2

PMID 35497303

Authors

Jian Zhang

Qiyue Zhang

He Li

Xinwei Chen

Wanlu Liu

Xinqi Liu

Affiliations

Soon will be listed here.

Abstract

This paper is aimed at purifying and identifying selenium (Se)-containing antioxidative peptides from Se-enriched soybean peptides (SSP). In this work, the SSP was separated into five fractions (F1 to F5). Fraction F4, displaying the highest antioxidative activity, was further separated, and sub-fractions F4-1 to F4-5 were selected for antioxidative activity evaluation using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzo-thiazoline-6-sulphonic acid)diammonium salt (ABTS), and OH radical scavenging assays. The Se-containing antioxidative peptides with sequence Ser-SeC-Ala-His-Lys (SSeCAHK) were identified in sub-fraction F4-1 and chemically synthesized. This Se-containing pentapeptide showed a preventive effect against hydrogen peroxide (HO)-induced oxidative stress in HepG2 cells. Pretreating the cells for 2 h with SSeCAHK (0.13-0.50 mg mL) induced strong intracellular, reactive oxygen species (ROS) scavenging activity while preventing a decrease in reduced glutathione (GSH) and an increase in malondialdehyde (MDA). Therefore, SSeCAHK treatment improved HO-induced oxidative stress in HepG2 cells, demonstrating the significant potential of SSeCAHK as a natural antioxidative functional material for dietary supplementation.

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