Electrochemical Detection of C-reactive Protein Using Functionalized Iridium Nanoparticles/graphene Oxide As a Tag

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 May 2

PMID 35497247

Authors

Yuchan Ma

Jiao Yang

Tong Yang

Yan Deng

Mengqiao Gu

Min Wang

Rong Hu

Yunhui Yang

Affiliations

Soon will be listed here.

Abstract

C-reactive protein (CRP) has become a recognized indicator of inflammation. CRP concentration in serum is an important indicator for monitoring early heart damage, and it is also a newly discovered coronary heart disease-associated inflammatory factor. A conductive nano-hybrid material composed of Au NPs and ionic liquid functionalized molybdenum disulfide (Au NPs/IL-MoS) was prepared and utilized to immobilize primary CRP antibodies. Subsequently, 1,5-diaminonaphthalene (DN) was adsorbed onto graphene oxide (GO) through π-π stacking, which was used to load iridium nanoparticles (Ir NPs) as a tag to label secondary CRP antibodies. The large surface area of Au NPs/IL-MoS and the excellent electrocatalytic properties of Ir NPs/GO-DN toward the reduction of HO resulted in a highly sensitive assay for CRP antigens. This immunosensor exhibited wide linear ranges from 0.01 to 100 ng mL and a lower detection of limit of 3.3 pg mL (S/N = 3). This CRP immunosensor can be applied in real serum sample analysis with satisfactory results, indicating that the immunosensor has potential applications in biomedical detection.

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