Utilizing Chemically Induced Dimerization of FKBP to Analyze Endocytosis by Live-cell Imaging in Budding Yeast

Overview

Journal STAR Protoc

Publisher Cell Press

Specialties Biology
Biomedical Engineering
Science

Date 2022 May 2

PMID 35496798

Authors

Andrew K Lamb

Santiago M Di Pietro

Affiliations

Soon will be listed here.

Abstract

Chemically induced dimerization (CID) is a useful tool for artificially inducing protein-protein interactions. Although CID has been used extensively for live-cell microscopy applications in mammalian systems, it is rarely utilized in yeast cell biology studies. Here, we present a step-by-step protocol for the utilization of a CID system in live-cell microscopy experiments of budding yeast endocytosis. While focusing on the study of endocytosis, this protocol framework is adaptable to the study of other cellular processes in . For complete details on the use and execution of this protocol, please refer to Lamb et al. (2021).

Citing Articles

Mechanism of actin capping protein recruitment and turnover during clathrin-mediated endocytosis.

Lamb A, Fernandez A, Eadaim A, Johnson K, Di Pietro S J Cell Biol. 2023; 223(1).

PMID: 37966720 PMC: 10651396. DOI: 10.1083/jcb.202306154.

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