Low Level of PALMD Contributes to the Metastasis of Uveal Melanoma

Overview

Journal Front Oncol

Specialty Oncology

Date 2022 May 2

PMID 35494064

Authors

Min-Yun Cai

Yue-Li Xu

Hua Rong

Hai Yang

Affiliations

Soon will be listed here.

Abstract

Uveal melanoma (UM) is a highly aggressive disease. There is an urgent need to develop the metastasis prediction markers of UM. This study aims to detect the key role of PALMD in UM metastasis. Transcriptome sequencing results of 2 sets of UM metastatic samples (GSE22138 and GSE156877) were downloaded from the Gene Expression Omnibus (GEO), and 18 overlapping differentially expressed genes were screened out, including PALMD. PALMD was significantly underexpressed in metastatic UM tissue. Low expression of PALMD was associated with poor prognosis in UM patients. The decreased expression of PALMD promoted the invasion and migration of 92-1 and Mel270 cells, while the high expression of PALMD inhibited the invasion and migration of UM cells. Furthermore, the levels of matrix metallopeptidase (MMP) 2 and MMP9 increased after transfection of siRNAs specifically targeting PALMD, whereas the levels of MMP2 and MMP9 were decreased after PALMD overexpression. However, PALMD did not affect the proliferation of UM cells. In addition, ZNF263 promoted the transcription of PALMD through the putative binding sequence using the JASPAR database, luciferase reporter gene analysis and chromatin immunoprecipitation assay. In summary, the expression of PALMD regulated by ZNF263 plays an important role in UM metastasis.

Citing Articles

Integrated machine learning and Mendelian randomization reveal PALMD as a prognostic biomarker for nonspecific orbital inflammation.

Wu Z, Liu X, Tan K, Yao X, Peng Q Sci Rep. 2024; 14(1):24020.

PMID: 39402101 PMC: 11473641. DOI: 10.1038/s41598-024-74409-1.

Machine Learning Methods for Gene Selection in Uveal Melanoma.

Reggiani F, El Rashed Z, Petito M, Pfeffer M, Morabito A, Tanda E Int J Mol Sci. 2024; 25(3).

PMID: 38339073 PMC: 10855534. DOI: 10.3390/ijms25031796.

Linking Gene Fusions to Bone Marrow Failure and Malignant Transformation in Dyskeratosis Congenita.

Gullulu O, Mayer B, Toplek F Int J Mol Sci. 2024; 25(3).

PMID: 38338888 PMC: 10855549. DOI: 10.3390/ijms25031606.

References

Edgar R, Domrachev M, Lash A . Gene Expression Omnibus: NCBI gene expression and hybridization array data repository. Nucleic Acids Res. 2001; 30(1):207-10. PMC: 99122. DOI: 10.1093/nar/30.1.207. View

Bosse Y, Mathieu P, Theriault S . PALMD as a novel target for calcific aortic valve stenosis. Curr Opin Cardiol. 2019; 34(2):105-111. DOI: 10.1097/HCO.0000000000000605. View

Hsu C, Hsu Y, Tsai Y, Shieh F, Huang S, Wan L . Emodin inhibits the growth of hepatoma cells: finding the common anti-cancer pathway using Huh7, Hep3B, and HepG2 cells. Biochem Biophys Res Commun. 2009; 392(4):473-8. DOI: 10.1016/j.bbrc.2009.10.153. View

Huang D, Wang X, Liu Y, Huang Z, Hu X, Hu W . Multi-omic analysis suggests tumor suppressor genes evolved specific promoter features to optimize cancer resistance. Brief Bioinform. 2021; 22(5). DOI: 10.1093/bib/bbab040. View

Chen J, Qian Z, Li F, Li J, Lu Y . Integrative Analysis of Microarray Data to Reveal Regulation Patterns in the Pathogenesis of Hepatocellular Carcinoma. Gut Liver. 2016; 11(1):112-120. PMC: 5221868. DOI: 10.5009/gnl16063. View

Dogrusoz M, Jager M, Damato B . Uveal Melanoma Treatment and Prognostication. Asia Pac J Ophthalmol (Phila). 2017; 6(2):186-196. DOI: 10.22608/APO.201734. View

Smit K, Boers R, Vaarwater J, Boers J, Brands T, Mensink H . Genome-wide aberrant methylation in primary metastatic UM and their matched metastases. Sci Rep. 2022; 12(1):42. PMC: 8742000. DOI: 10.1038/s41598-021-03964-8. View

Nie Y, Chen H, Guo C, Yuan Z, Zhou X, Zhang Y . Palmdelphin promotes myoblast differentiation and muscle regeneration. Sci Rep. 2017; 7:41608. PMC: 5288731. DOI: 10.1038/srep41608. View

Chignon A, Rosa M, Boulanger M, Argaud D, Devillers R, Bon-Baret V . Enhancer-associated aortic valve stenosis risk locus 1p21.2 alters NFATC2 binding site and promotes fibrogenesis. iScience. 2021; 24(3):102241. PMC: 7970363. DOI: 10.1016/j.isci.2021.102241. View

10.

Yang L, Feng S, Yang Y . Identification of transcription factors (TFs) and targets involved in the cholangiocarcinoma (CCA) by integrated analysis. Cancer Gene Ther. 2016; 23(12):439-445. DOI: 10.1038/cgt.2016.64. View

11.

Dashzeveg N, Taira N, Lu Z, Kimura J, Yoshida K . Palmdelphin, a novel target of p53 with Ser46 phosphorylation, controls cell death in response to DNA damage. Cell Death Dis. 2014; 5:e1221. PMC: 4047856. DOI: 10.1038/cddis.2014.176. View

12.

Kalebic N, Gilardi C, Stepien B, Wilsch-Brauninger M, Long K, Namba T . Neocortical Expansion Due to Increased Proliferation of Basal Progenitors Is Linked to Changes in Their Morphology. Cell Stem Cell. 2019; 24(4):535-550.e9. DOI: 10.1016/j.stem.2019.02.017. View

13.

Shain A, Bagger M, Yu R, Chang D, Liu S, Vemula S . The genetic evolution of metastatic uveal melanoma. Nat Genet. 2019; 51(7):1123-1130. PMC: 6632071. DOI: 10.1038/s41588-019-0440-9. View

14.

Theriault S, Gaudreault N, Lamontagne M, Rosa M, Boulanger M, Messika-Zeitoun D . A transcriptome-wide association study identifies PALMD as a susceptibility gene for calcific aortic valve stenosis. Nat Commun. 2018; 9(1):988. PMC: 5840407. DOI: 10.1038/s41467-018-03260-6. View

15.

Bao R, Surriga O, Olson D, Allred J, Strand C, Zha Y . Transcriptional analysis of metastatic uveal melanoma survival nominates NRP1 as a therapeutic target. Melanoma Res. 2020; 31(1):27-37. PMC: 7755667. DOI: 10.1097/CMR.0000000000000701. View

16.

Helgadottir A, Thorleifsson G, Gretarsdottir S, Stefansson O, Tragante V, Thorolfsdottir R . Genome-wide analysis yields new loci associating with aortic valve stenosis. Nat Commun. 2018; 9(1):987. PMC: 5840367. DOI: 10.1038/s41467-018-03252-6. View

17.

Xu Y, Han W, Xu W, Wang Y, Yang X, Nie H . Identification of differentially expressed genes and functional annotations associated with metastases of the uveal melanoma. J Cell Biochem. 2019; 120(11):19202-19214. DOI: 10.1002/jcb.29250. View

18.

Harbour J . Therapeutic Escape in Gαq-mutant Uveal Melanoma: It's a FAK. Clin Cancer Res. 2021; 27(11):2967-2969. PMC: 8172474. DOI: 10.1158/1078-0432.CCR-21-0567. View

19.

Jang G, Crabb J, Hu B, Willard B, Kalirai H, Singh A . Proteomics of Primary Uveal Melanoma: Insights into Metastasis and Protein Biomarkers. Cancers (Basel). 2021; 13(14). PMC: 8307952. DOI: 10.3390/cancers13143520. View

20.

Li C, Tang Z, Zhang W, Ye Z, Liu F . GEPIA2021: integrating multiple deconvolution-based analysis into GEPIA. Nucleic Acids Res. 2021; 49(W1):W242-W246. PMC: 8262695. DOI: 10.1093/nar/gkab418. View