Applicability of Electro-osmotic Flow for the Analysis of the Surface Zeta Potential

The analysis of the surface zeta potential (SZP) opens up new possibilities in the characterization of various materials used for scientific or industrial applications. It provides at the same time insight into the material surface chemistry and elucidates the interactions with charged species in the aqueous test solution. For this purpose, an accurate, reliable and repeatable analysis of the SZP is the key factor. This work focuses on a detailed and systematic comparison of two electrokinetic techniques, the mapping of the electro-osmotic flow (EOF) and the measurement of the streaming potential (SP), for the surface zeta potential (SZP) determination of several materials with varying properties. Both techniques have advantages as well as drawbacks. The applicability of latex polymer material and inorganic tracer particles at varying ionic strength, the interaction between oppositely charged tracer particles and solid surfaces, the assessment of the pH dependence of the SZP and the isoelectric point (IEP), and the effects of sample porosity and conductance have been investigated. Although in some cases the EOF method gives a SZP similar to the streaming potential measurement, especially when the tracer particle exhibits the same charge as the solid surface, it was revealed that reliable results were only obtained with the streaming potential and streaming current method. Several obstacles such as elevated conductivity at higher ionic strength, the applied voltage for the EM measurement, and the nature of tracer particles lower the accuracy and reliability of the SZP determined by the EOF method. It was shown that the EOF method is not applicable to oppositely charged surface and tracer particles and also limited to low salinity conditions especially when using polymeric tracer particles. Although the EOF method does not require the formation of a capillary flow channel, it disables a non-destructive SZP of fragile or valuable samples, such as QCM-D sensors, in comparison to the SP approach.