Controlling the Adsorption of Osteopontin for Mediating Cell Behaviour by Using Self-assembled Monolayers with Varying Surface Chemistry

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 May 2

PMID 35492794

Authors

Zhuoying Chen

Yan Fan

Lin Wang

Zhengqi Bian

Lijing Hao

Affiliations

Soon will be listed here.

Abstract

Osteopontin (OPN) is an important protein for mediating cell behaviour on biomaterials. However, the interactions between the chemical groups on the biomaterial surface and OPN still need to be further clarified, which has restricted the application of OPN in biomaterial functionalization. In the present study, we developed different self-assembled monolayers (SAMs) with specific chemical groups, including SAMs-OH, SAMs-OEG, SAMs-COOH, SAMs-NH, and SAMs-POH, to study the behavior of OPN on these SAMs. The results showed that SAMs-NH could strongly adsorb OPN, and the amount of protein was highest on this material. Meanwhile, the lowest amount of OPN was present on SAMs-OEG. Interestingly, the unit-mass trend of bound OPN monoclonal antibodies (mAbs) on the SAMs was opposite to the OPN adsorption trend: lowest on SAMs-NH but highest on SAMs-OEG. cell assay results showed that () on SAMs-COOH, SAMs-NH, and SAMs-POH with pre-adsorbed OPN showed promoted behaviour, in terms of spreading, viability, and the expression levels of α and β genes, compared with the other two SAMs, demonstrating the higher bioactivity of the adsorbed OPN. We believe that our findings will have great potential for developing OPN-activated biomaterials.

Citing Articles

Surface modifications of biomaterials in different applied fields.

Hu X, Wang T, Li F, Mao X RSC Adv. 2023; 13(30):20495-20511.

PMID: 37435384 PMC: 10331796. DOI: 10.1039/d3ra02248j.

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