The Antimicrobial Systems of Promote Niche Competition in Pig Tonsils

Overview

Journal Virulence

Specialty Microbiology

Date 2022 Apr 28

PMID 35481413

Authors

Zijing Liang

Huizhen Wu

Chen Bian

Hao Chen

Yanling Shen

Xueping Gao

Jiale Ma

Huochun Yao

Liping Wang

Zongfu Wu

Affiliations

Soon will be listed here.

Abstract

can cause severe infections in pigs and humans. The tonsils of pigs are major niches for , and different serotypes of can be found in the same tonsil. Pig tonsil colonization by is believed to be an important source of infection for humans and pigs. However, how competes for a stable tonsil niche is unknown. Here, we found that strain WUSS351, isolated from a healthy pig tonsil, is virulent and multidrug-resistant. The ABC transporter system SstFEG, conferring resistance to bacitracin, was reported to confer a competitive survival advantage . In addition, strain WUSS351 has several antimicrobial systems, including a novel type VII secretion system (T7SS), lantibiotic bacteriocin, and lactococcin972-like bacteriocin Lcn351. Bacterial competition experiments demonstrated T7SS-mediated cell contact-dependent antagonism of . Antibacterial activity analysis and 16S rRNA gene sequencing of the culture-independent and culture-dependent pig tonsillar microbiome revealed that Lcn351 mainly targets , one of the core microbiomes in pig tonsils. Taken together, our results revealed the mechanism of the stable persistence of in the tonsil niche, which might have important implications for epidemiology, potentially influencing strain prevalence and disease progression.

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References

Kumar S, Stecher G, Li M, Knyaz C, Tamura K . MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018; 35(6):1547-1549. PMC: 5967553. DOI: 10.1093/molbev/msy096. View

Whitney J, Peterson S, Kim J, Pazos M, Verster A, Radey M . A broadly distributed toxin family mediates contact-dependent antagonism between gram-positive bacteria. Elife. 2017; 6. PMC: 5555719. DOI: 10.7554/eLife.26938. View

Huang J, Sun J, Wu Y, Chen L, Duan D, Lv X . Identification and pathogenicity of an XDR Streptococcus suis isolate that harbours the phenicol-oxazolidinone resistance genes optrA and cfr, and the bacitracin resistance locus bcrABDR. Int J Antimicrob Agents. 2019; 54(1):43-48. DOI: 10.1016/j.ijantimicag.2019.04.003. View

Huang J, Ma J, Shang K, Hu X, Liang Y, Li D . Evolution and Diversity of the Antimicrobial Resistance Associated Mobilome in : A Probable Mobile Genetic Elements Reservoir for Other Streptococci. Front Cell Infect Microbiol. 2016; 6:118. PMC: 5053989. DOI: 10.3389/fcimb.2016.00118. View

Ferrando M, de Greeff A, van Rooijen W, Stockhofe-Zurwieden N, Nielsen J, Wichgers Schreur P . Host-pathogen Interaction at the Intestinal Mucosa Correlates With Zoonotic Potential of Streptococcus suis. J Infect Dis. 2014; 212(1):95-105. PMC: 4462715. DOI: 10.1093/infdis/jiu813. View

Liang P, Wang M, Gottschalk M, Vela A, Estrada A, Wang J . Genomic and pathogenic investigations of serotype 7 population derived from a human patient and pigs. Emerg Microbes Infect. 2021; 10(1):1960-1974. PMC: 8525962. DOI: 10.1080/22221751.2021.1988725. View

Zeghouf M, Li J, Butland G, Borkowska A, Canadien V, Richards D . Sequential Peptide Affinity (SPA) system for the identification of mammalian and bacterial protein complexes. J Proteome Res. 2004; 3(3):463-8. DOI: 10.1021/pr034084x. View

Li M, Zhang B, Li J, Sun L . Sil: a Streptococcus iniae bacteriocin with dual role as an antimicrobial and an immunomodulator that inhibits innate immune response and promotes S. iniae infection. PLoS One. 2014; 9(4):e96222. PMC: 4004548. DOI: 10.1371/journal.pone.0096222. View

Qiu X, Bai X, Lan R, Zheng H, Xu J . Novel Capsular Polysaccharide Loci and New Diagnostic Tools for High-Throughput Capsular Gene Typing in Streptococcus suis. Appl Environ Microbiol. 2016; 82(24):7102-7112. PMC: 5118921. DOI: 10.1128/AEM.02102-16. View

10.

Wu Z, Wu C, Shao J, Zhu Z, Wang W, Zhang W . The Streptococcus suis transcriptional landscape reveals adaptation mechanisms in pig blood and cerebrospinal fluid. RNA. 2014; 20(6):882-98. PMC: 4024642. DOI: 10.1261/rna.041822.113. View

11.

Houben D, Demangel C, van Ingen J, Perez J, Baldeon L, Abdallah A . ESX-1-mediated translocation to the cytosol controls virulence of mycobacteria. Cell Microbiol. 2012; 14(8):1287-98. DOI: 10.1111/j.1462-5822.2012.01799.x. View

12.

Goyette-Desjardins G, Auger J, Xu J, Segura M, Gottschalk M . Streptococcus suis, an important pig pathogen and emerging zoonotic agent-an update on the worldwide distribution based on serotyping and sequence typing. Emerg Microbes Infect. 2015; 3(6):e45. PMC: 4078792. DOI: 10.1038/emi.2014.45. View

13.

Wang X, Sun J, Bian C, Wang J, Liang Z, Shen Y . The population structure, antimicrobial resistance, and pathogenicity of Streptococcus suis cps31. Vet Microbiol. 2021; 259:109149. DOI: 10.1016/j.vetmic.2021.109149. View

14.

Taylor J, Gao X, Xu J, Holder M, Petrosino J, Kumar R . A type VII secretion system of Streptococcus gallolyticus subsp. gallolyticus contributes to gut colonization and the development of colon tumors. PLoS Pathog. 2021; 17(1):e1009182. PMC: 7815207. DOI: 10.1371/journal.ppat.1009182. View

15.

Darling A, Mau B, Perna N . progressiveMauve: multiple genome alignment with gene gain, loss and rearrangement. PLoS One. 2010; 5(6):e11147. PMC: 2892488. DOI: 10.1371/journal.pone.0011147. View

16.

Marti Nez B, Rodri Guez A, Suarez J . Lactococcin 972, a bacteriocin that inhibits septum formation in lactococci. Microbiology (Reading). 2000; 146 ( Pt 4):949-955. DOI: 10.1099/00221287-146-4-949. View

17.

Bertelli C, Laird M, Williams K, Lau B, Hoad G, Winsor G . IslandViewer 4: expanded prediction of genomic islands for larger-scale datasets. Nucleic Acids Res. 2017; 45(W1):W30-W35. PMC: 5570257. DOI: 10.1093/nar/gkx343. View

18.

Lai L, Dai J, Tang H, Zhang S, Wu C, Qiu W . Streptococcus suis serotype 9 strain GZ0565 contains a type VII secretion system putative substrate EsxA that contributes to bacterial virulence and a vanZ-like gene that confers resistance to teicoplanin and dalbavancin in Streptococcus agalactiae. Vet Microbiol. 2017; 205:26-33. DOI: 10.1016/j.vetmic.2017.04.030. View

19.

Quereda J, Dussurget O, Nahori M, Ghozlane A, Volant S, Dillies M . Bacteriocin from epidemic Listeria strains alters the host intestinal microbiota to favor infection. Proc Natl Acad Sci U S A. 2016; 113(20):5706-11. PMC: 4878514. DOI: 10.1073/pnas.1523899113. View

20.

Ngo T, Tran T, Tran T, Nguyen V, Campbell J, Pham H . Slaughterhouse pigs are a major reservoir of Streptococcus suis serotype 2 capable of causing human infection in southern Vietnam. PLoS One. 2011; 6(3):e17943. PMC: 3065462. DOI: 10.1371/journal.pone.0017943. View