Functional Differentiation of Gene Homologous Copies in α-Tocopherol Biosynthesis Revealed by CRISPR/Cas9 Editing

Overview

Journal Front Plant Sci

Date 2022 Apr 28

PMID 35481148

Authors

Haiyan Zhang

Yuqin Shi

Mengdan Sun

Xuezhi Hu

Mengyu Hao

Yu Shu

Xue-Rong Zhou

Qiong Hu

Chao Li

Desheng Mei

Affiliations

Soon will be listed here.

Abstract

Tocopherols are essential nutrients for human health known as vitamin E. Vitamin E deficiency can have a profound effect on human health, including the central nervous system and cardiovascular and immune protection. Multiple enzymatic steps are involved in the conversion between different forms of tocopherols. Among them, γ-tocopherol methyltransferase encoded by gene catalyzes the conversion of γ- to α-tocopherol or δ- to β-tocopherol isoforms. However, the gene copies and their functional contribution of homologs in were not elucidated. To this end, different mutation combinations of four putative homologous copies were generated by using CRISPR/Cas9 genome editing technology. Editing of those homologs led to a significant change of the α-tocopherol content and the ratio between α- and γ-tocopherol compared with wide-type control. Analysis of the different combinations of -edited homologs revealed that the contribution of the individual gene displayed obvious functional differentiation in α-tocopherol biosynthesis. Their contribution could be in order of (BnaC02G0331100ZS) > (BnaA02G0247300ZS) > (BnaA02G0154300ZS). Moreover, the and copies might have severe functional redundancies in α-tocopherol biosynthesis. Overall, this study systemically studied the different effects of homologs, which provided a theoretical basis for breeding high α-tocopherol content oilseed rape.

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