The Restricted Adhesion of Bone Marrow Mesenchymal Stem Cells by Stepped Structures on Surfaces of Hydroxyapatite

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 Apr 28

PMID 35481104

Authors

Jin Chen

Zhuo Huang

Fang Wang

Min Gong

Xueli Zhang

Yajing Wang

Zuquan Hu

Zhu Zeng

Yun Wang

Affiliations

Soon will be listed here.

Abstract

Currently, many researches have developed several strategies to design the surface structures of hydroxyapatite (HA), and have proved that the surface structures are pivotal in guiding the adhesion of bone marrow mesenchymal stem cells (BMSCs) as well as subsequent cellular behaviours. Most of these strategies, such as altering roughness and constructing surface patterning of HA, involve the construction of geometric topographies at the micro/nanoscale. However, besides geometric topographies, crystal defects are also important characteristics of surface structures and would alter many local physicochemical properties, which is critical for contact between cells and bioceramic surfaces. For the practical applications of crystal defects, a major hindrance is that crystal defects are usually unstable and easily eliminated during crystallization, which limits the large-scale fabrication of materials with crystal defects. In this work, given that stepped structures contain massive stable crystal defects on their step edges and kinks, we proposed a feasible and efficient method to fabricate HA dishes with stepped structures on their surfaces. First, plate-like HA mesocrystals were prepared from CaHPO topotactic transformation, and were shaped into HA dishes by vacuum-filtration. Then, a sintering process was applied to facilitate the formation of stepped structures on the surfaces. We demonstrated that the generation of stepped structures could restrict the adhesion of BMSCs and showed the restriction effect is highly correlated with the density of exposed stepped structures. This phenomenon is interesting and the construction of a cell adhesion model is robust and easy, the underlying mechanisms of which deserve further exploration. Furthermore, constructing stepped structures on surfaces may be a new useful strategy to regulate cell adhesion and could also cooperate with other methods that do not need change in the surface crystal structure.

References

Surmenev R, Surmeneva M, Ivanova A . Significance of calcium phosphate coatings for the enhancement of new bone osteogenesis--a review. Acta Biomater. 2013; 10(2):557-79. DOI: 10.1016/j.actbio.2013.10.036. View

Zhao C, Xia L, Zhai D, Zhang N, Liu J, Fang B . Designing ordered micropatterned hydroxyapatite bioceramics to promote the growth and osteogenic differentiation of bone marrow stromal cells. J Mater Chem B. 2020; 3(6):968-976. DOI: 10.1039/c4tb01838a. View

Zhang J, Huang F, Lin Z . Progress of nanocrystalline growth kinetics based on oriented attachment. Nanoscale. 2010; 2(1):18-34. DOI: 10.1039/b9nr00047j. View

Li D, Nielsen M, Lee J, Frandsen C, Banfield J, De Yoreo J . Direction-specific interactions control crystal growth by oriented attachment. Science. 2012; 336(6084):1014-8. DOI: 10.1126/science.1219643. View

Yang C, Zhao C, Wang X, Shi M, Zhu Y, Jing L . Stimulation of osteogenesis and angiogenesis by micro/nano hierarchical hydroxyapatite via macrophage immunomodulation. Nanoscale. 2019; 11(38):17699-17708. DOI: 10.1039/c9nr05730g. View

Yin X, Shi J, Niu X, Huang H, Wang X . Wedding Cake Growth Mechanism in One-Dimensional and Two-Dimensional Nanostructure Evolution. Nano Lett. 2015; 15(11):7766-72. DOI: 10.1021/acs.nanolett.5b04072. View

Zamir E, Katz M, Posen Y, Erez N, Yamada K, Katz B . Dynamics and segregation of cell-matrix adhesions in cultured fibroblasts. Nat Cell Biol. 2000; 2(4):191-6. DOI: 10.1038/35008607. View

Connelly J, Gautrot J, Trappmann B, Tan D, Donati G, Huck W . Actin and serum response factor transduce physical cues from the microenvironment to regulate epidermal stem cell fate decisions. Nat Cell Biol. 2010; 12(7):711-8. DOI: 10.1038/ncb2074. View

Lin H, Sohn J, Shen H, Langhans M, Tuan R . Bone marrow mesenchymal stem cells: Aging and tissue engineering applications to enhance bone healing. Biomaterials. 2018; 203:96-110. PMC: 6733253. DOI: 10.1016/j.biomaterials.2018.06.026. View

10.

Yang W, Han W, He W, Li J, Wang J, Feng H . Surface topography of hydroxyapatite promotes osteogenic differentiation of human bone marrow mesenchymal stem cells. Mater Sci Eng C Mater Biol Appl. 2015; 60:45-53. DOI: 10.1016/j.msec.2015.11.012. View

11.

Pajarinen J, Lin T, Gibon E, Kohno Y, Maruyama M, Nathan K . Mesenchymal stem cell-macrophage crosstalk and bone healing. Biomaterials. 2018; 196:80-89. PMC: 6028312. DOI: 10.1016/j.biomaterials.2017.12.025. View

12.

De Yoreo J, Gilbert P, Sommerdijk N, Penn R, Whitelam S, Joester D . CRYSTAL GROWTH. Crystallization by particle attachment in synthetic, biogenic, and geologic environments. Science. 2015; 349(6247):aaa6760. DOI: 10.1126/science.aaa6760. View

13.

Zaidel-Bar R, Cohen M, Addadi L, Geiger B . Hierarchical assembly of cell-matrix adhesion complexes. Biochem Soc Trans. 2004; 32(Pt3):416-20. DOI: 10.1042/BST0320416. View

14.

Etacheri V, Michlits G, Seery M, Hinder S, Pillai S . A highly efficient TiO(2-x)C(x) nano-heterojunction photocatalyst for visible light induced antibacterial applications. ACS Appl Mater Interfaces. 2013; 5(5):1663-72. DOI: 10.1021/am302676a. View

15.

Zhang H, De Yoreo J, Banfield J . A unified description of attachment-based crystal growth. ACS Nano. 2014; 8(7):6526-30. DOI: 10.1021/nn503145w. View

16.

Shen J, Wu T, Wang Q, Pan H . Molecular simulation of protein adsorption and desorption on hydroxyapatite surfaces. Biomaterials. 2007; 29(5):513-32. DOI: 10.1016/j.biomaterials.2007.10.016. View

17.

Ballestrem C, Hinz B, Imhof B, Wehrle-Haller B . Marching at the front and dragging behind: differential alphaVbeta3-integrin turnover regulates focal adhesion behavior. J Cell Biol. 2002; 155(7):1319-32. PMC: 2199321. DOI: 10.1083/jcb.200107107. View

18.

Wu C, Chen M, Xing C . Molecular understanding of conformational dynamics of a fibronectin module on rutile (110) surface. Langmuir. 2010; 26(20):15972-81. DOI: 10.1021/la103010c. View

19.

Tamimi F, Sheikh Z, Barralet J . Dicalcium phosphate cements: brushite and monetite. Acta Biomater. 2011; 8(2):474-87. DOI: 10.1016/j.actbio.2011.08.005. View

20.

Liao C, Zhou J . Replica-exchange molecular dynamics simulation of basic fibroblast growth factor adsorption on hydroxyapatite. J Phys Chem B. 2014; 118(22):5843-52. DOI: 10.1021/jp501463r. View