Absolute Content Determination by Quantitative NMR (qNMR) Spectroscopy: a Curious Case of Aldosterone

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 Apr 28

PMID 35479823

Authors

Neeraj Singh

Judith Taibon

Stephan Pongratz

Christian Geletneky

Affiliations

Soon will be listed here.

Abstract

Quantitative NMR spectroscopy has been utilized to calculate the absolute content (g g) of aldosterone, which is necessary for electrolyte balance and blood pressure regulation, in commercially available materials. Explanations have been provided for many signals observed in the HNMR spectrum, false interpretation of which can have significant effects if such a value is utilized for the primary calibrators in ID-LC-MS/MS ('gold standard') reference methods in clinical chemistry.

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