Design and Synthesis of Some New Furan-Based Derivatives and Evaluation of In Vitro Cytotoxic Activity

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Apr 23

PMID 35458804

Authors

Syed Nasir Abbas Bukhari

Hasan Ejaz

Mervat A Elsherif

Kashaf Junaid

Islam Zaki

Reham E Masoud

Affiliations

Soon will be listed here.

Abstract

New furan-based derivatives have been, designed, synthesized, and evaluated for their cytotoxic and tubulin polymerization inhibitory activities. DNA flow cytometric study of pyridine carbohydrazide and -phenyl triazinone demonstrated G/M phase cell cycle disruptions. Accumulation of cells in the pre-G1 phase and positive annexin V/PI staining, which may be caused by degeneration or fragmentation of the genetic components, suggested that cell death occurs via an apoptotic cascade. Furthermore, compounds and had a strong pro-apoptotic impact through inducing the intrinsic mitochondrial mechanism of apoptosis. This mechanistic route was verified by an ELISA experiment that indicated a considerable rise in the levels of p53 and Bax and a drop in the level of Bcl-2 when compared with the control.

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