» Articles » PMID: 35457268

and Play Redundant Roles in Wax Synthesis

Overview
Journal Int J Mol Sci
Publisher MDPI
Date 2022 Apr 23
PMID 35457268
Authors
Affiliations
Soon will be listed here.
Abstract

as components of a fatty acid elongase (FAE) complex, play key roles in determining the chain length of very-long-chain fatty acids (VLCFAs). taking a predominate role during the elongation from C26 to C28, is well known to play an important role in wax synthesis. is one paralog of and its role in wax synthesis remains unknown. Wax phenotype analysis showed that in mutants, the total amounts of wax components derived from carbon 32 (C32) and C34 were apparently decreased in leaves, and those of C26 to C32 derivatives were obviously decreased in flowers. Heterologous yeast expression analysis showed that alone displayed specificity towards C24 to C28 acids, and its coordination with and catalyzed the elongation of acids exceeding C28, especially displaying higher activity towards C28 acids than . BiLC experiments identified that physically interacts with and . Wax phenotype analysis of different organs in and single or double mutants showed that mutation causes greater effects on the wax synthesis than mutation in the tested organs, and simultaneous repression of both protein activities caused additive effects, suggesting that during the wax biosynthesis process, and play redundant roles, among which plays a major role. In addition, simultaneous mutations of two genes nearly block drought-induced wax production, indicating that the reactions catalyzed by and play a critical role in the wax biosynthesis in response to drought.

Citing Articles

A β-ketoacyl-CoA synthase encoded by DDP1 controls rice anther dehiscence and pollen fertility by maintaining lipid homeostasis in the tapetum.

Xu Y, Zhou S, Tian J, Zhao W, Wei J, He J Theor Appl Genet. 2024; 138(1):1.

PMID: 39627399 DOI: 10.1007/s00122-024-04786-8.


Arabidopsis DREB26/ERF12 and its close relatives regulate cuticular wax biosynthesis under drought stress condition.

Urano K, Oshima Y, Ishikawa T, Kajino T, Sakamoto S, Sato M Plant J. 2024; 120(5):2057-2075.

PMID: 39466828 PMC: 11629741. DOI: 10.1111/tpj.17100.


BnUC1 Is a Key Regulator of Epidermal Wax Biosynthesis and Lipid Transport in .

Ni F, Yang M, Chen J, Guo Y, Wan S, Zhao Z Int J Mol Sci. 2024; 25(17).

PMID: 39273481 PMC: 11394786. DOI: 10.3390/ijms25179533.


Gas Chromatography-Mass Spectrometry Metabolite Analysis Combined with Transcriptomics Reveals Genes Involved in Wax Biosynthesis in L.

Xing J, Xu H, Zhu M, Zhang Y, Bai M, Zhou X Int J Mol Sci. 2024; 25(11).

PMID: 38892292 PMC: 11173144. DOI: 10.3390/ijms25116106.


A chromosome level genome assembly of Pseudoroegneria Libanotica reveals a key Kcs gene involves in the cuticular wax elongation for drought resistance.

Zhai X, Wu D, Chen C, Yang X, Cheng S, Sha L BMC Genomics. 2024; 25(1):253.

PMID: 38448864 PMC: 10916072. DOI: 10.1186/s12864-024-10140-5.


References
1.
Patwari P, Salewski V, Gutbrod K, Kreszies T, Dresen-Scholz B, Peisker H . Surface wax esters contribute to drought tolerance in Arabidopsis. Plant J. 2019; 98(4):727-744. DOI: 10.1111/tpj.14269. View

2.
Kachroo A, Kachroo P . Fatty Acid-derived signals in plant defense. Annu Rev Phytopathol. 2009; 47:153-76. DOI: 10.1146/annurev-phyto-080508-081820. View

3.
Millar A, Kunst L . Very-long-chain fatty acid biosynthesis is controlled through the expression and specificity of the condensing enzyme. Plant J. 1997; 12(1):121-31. DOI: 10.1046/j.1365-313x.1997.12010121.x. View

4.
Paul S, Gable K, Beaudoin F, Cahoon E, Jaworski J, Napier J . Members of the Arabidopsis FAE1-like 3-ketoacyl-CoA synthase gene family substitute for the Elop proteins of Saccharomyces cerevisiae. J Biol Chem. 2006; 281(14):9018-29. DOI: 10.1074/jbc.M507723200. View

5.
Wang X, Guan Y, Zhang D, Dong X, Tian L, Qu L . A β-Ketoacyl-CoA Synthase Is Involved in Rice Leaf Cuticular Wax Synthesis and Requires a CER2-LIKE Protein as a Cofactor. Plant Physiol. 2016; 173(2):944-955. PMC: 5291035. DOI: 10.1104/pp.16.01527. View