Insights into Mechanisms of Damage Recognition and Catalysis by APE1-like Enzymes

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Apr 23

PMID 35457179

Authors

Anatoly A Bulygin

Olga S Fedorova

Nikita A Kuznetsov

Affiliations

Soon will be listed here.

Abstract

Apurinic/apyrimidinic (AP) endonucleases are the key DNA repair enzymes in the base excision repair (BER) pathway, and are responsible for hydrolyzing phosphodiester bonds on the 5' side of an AP site. The enzymes can recognize not only AP sites but also some types of damaged bases, such as 1,-ethenoadenosine, α-adenosine, and 5,6-dihydrouridine. Here, to elucidate the mechanism underlying such a broad substrate specificity as that of AP endonucleases, we performed a computational study of four homologous APE1-like endonucleases: insect () Rrp1, amphibian () APE1 (xAPE1), fish () APE1 (zAPE1), and human APE1 (hAPE1). The contact between the amino acid residues of the active site of each homologous APE1-like enzyme and the set of damaged DNA substrates was analyzed. A comparison of molecular dynamic simulation data with the known catalytic efficiency of these enzymes allowed us to gain a deep insight into the differences in the efficiency of the cleavage of various damaged nucleotides. The obtained data support that the amino acid residues within the "damage recognition" loop containing residues Asn222-Ala230 significantly affect the catalytic-complex formation. Moreover, every damaged nucleotide has its unique position and a specific set of interactions with the amino acid residues of the active site.

Citing Articles

The Trajectory of Damaged-Base Eversion into the Active Site of Apurinic/Apyrimidinic Endonuclease APE1 Regulates This Enzyme's Substrate Specificity.

Bulygin A, Kuznetsov N Int J Mol Sci. 2024; 25(22).

PMID: 39596352 PMC: 11595180. DOI: 10.3390/ijms252212287.

An Insight into the Mechanism of DNA Cleavage by DNA Endonuclease from the Hyperthermophilic Archaeon .

Davletgildeeva A, Kuznetsova A, Ishchenko A, Saparbaev M, Kuznetsov N Int J Mol Sci. 2024; 25(16).

PMID: 39201583 PMC: 11354406. DOI: 10.3390/ijms25168897.

Inner Amino Acid Contacts Are Key Factors of Multistage Structural Rearrangements of DNA and Affect Substrate Specificity of Apurinic/Apyrimidinic Endonuclease APE1.

Bulygin A, Syryamina V, Kuznetsova A, Novopashina D, Dzuba S, Kuznetsov N Int J Mol Sci. 2023; 24(14).

PMID: 37511233 PMC: 10380840. DOI: 10.3390/ijms241411474.

Special Issue on "Enzymes as Biocatalysts: Current Research Trends and Applications".

Sousa S Int J Mol Sci. 2022; 23(24).

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