Fatty Acids and Metabolomic Composition of Follicular Fluid Collected from Environments Associated with Good and Poor Oocyte Competence in Goats

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Apr 23

PMID 35456957

Authors

Dolors Izquierdo

Montserrat Roura

Miriam Perez-Trujillo

Sandra Soto-Heras

Maria-Teresa Paramio

Affiliations

Soon will be listed here.

Abstract

In goats, embryo oocyte competence is affected by follicle size regardless the age of the females. In previous studies we have found differences in blastocyst development between oocytes coming of small (<3 mm) and large follicles (>3 mm) in prepubertal (1−2 months-old) goats. Oocyte competence and Follicular Fluid (FF) composition changes throughout follicle growth. The aim of this study was to analyze Fatty Acids (FAs) composition and metabolomic profiles of FF recovered from small and large follicles of prepubertal goats and follicles of adult goats. FAs were analyzed by chromatography and metabolites by 1H-Nuclear Magnetic Resonance (1H-NMR) Spectrometry. The results showed important differences between adult and prepubertal follicles: (a) the presence of α,β-glucose in adult and no detection in prepubertal; (b) lactate, -N-(CH3)3 groups and inositol were higher in prepubertal (c) the percentage of Linolenic Acid, Total Saturated Fatty Acids and n-3 PUFAs were higher in adults; and (d) the percentage of Linoleic Acid, total MUFAs, PUFAs, n-6 PUFAs and n-6 PUFAs: n-3 PUFAs ratio were higher in prepubertal goats. Not significant differences were found in follicle size of prepubertal goats, despite the differences in oocyte competence for in vitro embryo production.

Citing Articles

Metabolomic profile of dromedary camel follicular fluid during the breeding and non-breeding seasons.

Abdoon A, Soliman S, Hussein N, Haggag S, El-Sanea A, Abdel-Hamid A Sci Rep. 2025; 15(1):8923.

PMID: 40087336 DOI: 10.1038/s41598-025-91710-9.

β-Nicotinamide Mononucleotide Reduces Oxidative Stress and Improves Steroidogenesis in Granulosa Cells Associated with Sheep Prolificacy via Activating AMPK Pathway.

Cai Y, Yang H, Xu H, Li S, Zhao B, Wang Z Antioxidants (Basel). 2025; 14(1).

PMID: 39857368 PMC: 11762531. DOI: 10.3390/antiox14010034.

Efficacy of Fat Supplements with Different Unsaturated/Saturated FA Ratios Undergoing First Postpartum Ovulation in Lactating Anovulatory Goats.

Silva C, Fernandes C, Alves J, Cavalcanti C, Oliveira F, Conde A Vet Sci. 2025; 12(1).

PMID: 39852935 PMC: 11768978. DOI: 10.3390/vetsci12010060.

Transcriptomic and metabolomic data of goat ovarian and uterine tissues during sexual maturation.

Wang Y, Wang J, Li Q, Xuan R, Guo Y, He P Sci Data. 2024; 11(1):777.

PMID: 39003290 PMC: 11246480. DOI: 10.1038/s41597-024-03565-w.

Growth hormone treatment improves the development of follicles and oocytes in prepubertal lambs.

Liu K, Zhang L, Qi Q, Li J, Yan F, Hou J J Ovarian Res. 2023; 16(1):132.

PMID: 37408062 PMC: 10324218. DOI: 10.1186/s13048-023-01209-y.

References

Martino A, Mogas T, Palomo M, Paramio M . Meiotic competence of prepubertal goat oocytes. Theriogenology. 1994; 41(4):969-80. DOI: 10.1016/0093-691x(94)90512-h. View

Papaleo E, Unfer V, Baillargeon J, Chiu T . Contribution of myo-inositol to reproduction. Eur J Obstet Gynecol Reprod Biol. 2009; 147(2):120-3. DOI: 10.1016/j.ejogrb.2009.09.008. View

Bracewell-Milnes T, Saso S, Abdalla H, Nikolau D, Norman-Taylor J, Johnson M . Metabolomics as a tool to identify biomarkers to predict and improve outcomes in reproductive medicine: a systematic review. Hum Reprod Update. 2017; 23(6):723-736. DOI: 10.1093/humupd/dmx023. View

Anguita B, Paramio M, Jimenez-Macedo A, Morato R, Mogas T, Izquierdo D . Total RNA and protein content, Cyclin B1 expression and developmental competence of prepubertal goat oocytes. Anim Reprod Sci. 2007; 103(3-4):290-303. DOI: 10.1016/j.anireprosci.2006.12.018. View

Goodpaster A, Romick-Rosendale L, Kennedy M . Statistical significance analysis of nuclear magnetic resonance-based metabonomics data. Anal Biochem. 2010; 401(1):134-43. DOI: 10.1016/j.ab.2010.02.005. View

Bertevello P, Teixeira-Gomes A, Labas V, Cordeiro L, Blache M, Papillier P . MALDI-TOF Mass Spectrometry Revealed Significant Lipid Variations in Follicular Fluid and Somatic Follicular Cells but Not in Enclosed Oocytes between the Large Dominant and Small Subordinate Follicles in Bovine Ovary. Int J Mol Sci. 2020; 21(18). PMC: 7554725. DOI: 10.3390/ijms21186661. View

Orsi N, Gopichandran N, Leese H, Picton H, Harris S . Fluctuations in bovine ovarian follicular fluid composition throughout the oestrous cycle. Reproduction. 2005; 129(2):219-28. DOI: 10.1530/rep.1.00460. View

Crozet N, Ahmed-Ali M, Dubos M . Developmental competence of goat oocytes from follicles of different size categories following maturation, fertilization and culture in vitro. J Reprod Fertil. 1995; 103(2):293-8. DOI: 10.1530/jrf.0.1030293. View

Romaguera R, Moll X, Morato R, Roura M, Palomo M, Catala M . Prepubertal goat oocytes from large follicles result in similar blastocyst production and embryo ploidy than those from adult goats. Theriogenology. 2011; 76(1):1-11. DOI: 10.1016/j.theriogenology.2010.12.014. View

10.

Nicholson J, Foxall P, Spraul M, Farrant R, Lindon J . 750 MHz 1H and 1H-13C NMR spectroscopy of human blood plasma. Anal Chem. 1995; 67(5):793-811. DOI: 10.1021/ac00101a004. View

11.

Roura M, Catala M, Soto-Heras S, Hammami S, Izquierdo D, Fouladi-Nashta A . Linoleic (LA) and linolenic (ALA) acid concentrations in follicular fluid of prepubertal goats and their effect on oocyte in vitro maturation and embryo development. Reprod Fertil Dev. 2017; 30(2):286-296. DOI: 10.1071/RD17174. View

12.

Paramio M, Izquierdo D . Current status of in vitro embryo production in sheep and goats. Reprod Domest Anim. 2014; 49 Suppl 4:37-48. DOI: 10.1111/rda.12334. View

13.

Chiu T, Rogers M, Law E, Briton-Jones C, Cheung L, Haines C . Follicular fluid and serum concentrations of myo-inositol in patients undergoing IVF: relationship with oocyte quality. Hum Reprod. 2002; 17(6):1591-6. DOI: 10.1093/humrep/17.6.1591. View

14.

Gandolfi F, Brevini T, Cillo F, Antonini S . Cellular and molecular mechanisms regulating oocyte quality and the relevance for farm animal reproductive efficiency. Rev Sci Tech. 2005; 24(1):413-23. View

15.

Sinclair K, Lunn L, Kwong W, Wonnacott K, Linforth R, Craigon J . Amino acid and fatty acid composition of follicular fluid as predictors of in-vitro embryo development. Reprod Biomed Online. 2008; 16(6):859-68. DOI: 10.1016/s1472-6483(10)60153-8. View

16.

Richani D, Dunning K, Thompson J, Gilchrist R . Metabolic co-dependence of the oocyte and cumulus cells: essential role in determining oocyte developmental competence. Hum Reprod Update. 2020; 27(1):27-47. DOI: 10.1093/humupd/dmaa043. View

17.

Ginther O, Kot K . Follicular dynamics during the ovulatory season in goats. Theriogenology. 1994; 42(6):987-1001. DOI: 10.1016/0093-691x(94)90121-x. View

18.

Ghaffarilaleh V, Fouladi-Nashta A, Paramio M . Effect of α-linolenic acid on oocyte maturation and embryo development of prepubertal sheep oocytes. Theriogenology. 2014; 82(5):686-96. DOI: 10.1016/j.theriogenology.2014.05.027. View

19.

Russo M, Forte G, Oliva M, Lagana A, Unfer V . Melatonin and Myo-Inositol: Supporting Reproduction from the Oocyte to Birth. Int J Mol Sci. 2021; 22(16). PMC: 8395120. DOI: 10.3390/ijms22168433. View

20.

Annes K, Muller D, Vilela J, Valente R, Caetano D, Cibin F . Influence of follicle size on bovine oocyte lipid composition, follicular metabolic and stress markers, embryo development and blastocyst lipid content. Reprod Fertil Dev. 2018; 31(3):462-472. DOI: 10.1071/RD18109. View