Effects of Rhizobia Isolated from Coffee Fields in the High Jungle Peruvian Region, Tested on L. Var. Red Kidney

Overview

Journal Microorganisms

Specialty Microbiology

Date 2022 Apr 23

PMID 35456872

Authors

Jesus Lirio-Paredes

Katty Ogata-Gutierrez

Doris Zuniga-Davila

Affiliations

Soon will be listed here.

Abstract

Soils in the high jungle region of Peru continuously face erosion due to heavy rain, which leads to significant nutrient losses. Leguminous plants may provide a sustainable solution to this problem due to their ability to fix atmospheric nitrogen with the help of symbiotic rhizospheric microbes that reside in their root nodules and help restore soil fertility. The aim of this study was to isolate native rhizobial strains that can form functional nodules in red kidney beans to help improve their growth, development, and yield in field conditions. strains were isolated from soil samples collected from coffee fields using bean plants as trap hosts. The strain RZC12 was selected because it showed good root nodule promotion and a number of PGPR (plant-growth-promoting rhizobacteria) attributes. In the field, bean plants inoculated with the strain RZC12 and co-cultivated with coffee plants produced approximately 21 nodules per plant, whereas control plants produced an average of 1 nodule each. The inoculation with RZC12 significantly increased plant length (72.7%), number of leaves (58.8%), fresh shoot weight (85.5%), dry shoot weight (78%), fresh root weight (85.7%), and dry root weight (82.5%), compared with the control. The dry pod weight produced by the plants inoculated with RZC12 was 3.8 g, whereas the control plants produced 2.36 g of pods. In conclusion, RZC12 is a promising strain that can be used in field conditions to improve the overall productivity of red kidney beans.

References

Santi C, Bogusz D, Franche C . Biological nitrogen fixation in non-legume plants. Ann Bot. 2013; 111(5):743-67. PMC: 3631332. DOI: 10.1093/aob/mct048. View

Gordon S, Weber R . COLORIMETRIC ESTIMATION OF INDOLEACETIC ACID. Plant Physiol. 1951; 26(1):192-5. PMC: 437633. DOI: 10.1104/pp.26.1.192. View

LARKIN M, Blackshields G, Brown N, Chenna R, McGettigan P, McWilliam H . Clustal W and Clustal X version 2.0. Bioinformatics. 2007; 23(21):2947-8. DOI: 10.1093/bioinformatics/btm404. View

Couzigou J, Zhukov V, Mondy S, Abu el Heba G, Cosson V, Ellis T . NODULE ROOT and COCHLEATA maintain nodule development and are legume orthologs of Arabidopsis BLADE-ON-PETIOLE genes. Plant Cell. 2012; 24(11):4498-510. PMC: 3531848. DOI: 10.1105/tpc.112.103747. View

Kawaka F, Dida M, Opala P, Ombori O, Maingi J, Osoro N . Symbiotic Efficiency of Native Rhizobia Nodulating Common Bean (Phaseolus vulgaris L.) in Soils of Western Kenya. Int Sch Res Notices. 2016; 2014:258497. PMC: 4897560. DOI: 10.1155/2014/258497. View

Weisburg W, Barns S, Pelletier D, Lane D . 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol. 1991; 173(2):697-703. PMC: 207061. DOI: 10.1128/jb.173.2.697-703.1991. View

Allen D, Cardinale B, Wynn-Thompson T . Plant biodiversity effects in reducing fluvial erosion are limited to low species richness. Ecology. 2016; 97(1):17-24. DOI: 10.1890/15-0800.1. View

Glickmann E, Dessaux Y . A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria. Appl Environ Microbiol. 1995; 61(2):793-6. PMC: 1388360. DOI: 10.1128/aem.61.2.793-796.1995. View

Nautiyal C . An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiol Lett. 1999; 170(1):265-70. DOI: 10.1111/j.1574-6968.1999.tb13383.x. View

10.

Lin M, Gresshoff P, Ferguson B . Systemic regulation of soybean nodulation by acidic growth conditions. Plant Physiol. 2012; 160(4):2028-39. PMC: 3510129. DOI: 10.1104/pp.112.204149. View

11.

Hawkins J, Oresnik I . The Rhizobium-Legume Symbiosis: Co-opting Successful Stress Management. Front Plant Sci. 2022; 12:796045. PMC: 8761673. DOI: 10.3389/fpls.2021.796045. View

12.

Frankowski K, Wilmowicz E, Kucko A, Zienkiewicz A, Zienkiewicz K, Kopcewicz J . Molecular cloning of the BLADE-ON-PETIOLE gene and expression analyses during nodule development in Lupinus luteus. J Plant Physiol. 2015; 179:35-9. DOI: 10.1016/j.jplph.2015.01.019. View

13.

Verbon E, Liberman L . Beneficial Microbes Affect Endogenous Mechanisms Controlling Root Development. Trends Plant Sci. 2016; 21(3):218-229. PMC: 4772406. DOI: 10.1016/j.tplants.2016.01.013. View

14.

Abhilash P, Dubey R, Tripathi V, Gupta V, Singh H . Plant Growth-Promoting Microorganisms for Environmental Sustainability. Trends Biotechnol. 2016; 34(11):847-850. DOI: 10.1016/j.tibtech.2016.05.005. View

15.

Jez J, Lee S, Sherp A . The next green movement: Plant biology for the environment and sustainability. Science. 2016; 353(6305):1241-4. DOI: 10.1126/science.aag1698. View

16.

Saitou N, Nei M . The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987; 4(4):406-25. DOI: 10.1093/oxfordjournals.molbev.a040454. View

17.

Bhardwaj D, Ansari M, Sahoo R, Tuteja N . Biofertilizers function as key player in sustainable agriculture by improving soil fertility, plant tolerance and crop productivity. Microb Cell Fact. 2014; 13:66. PMC: 4022417. DOI: 10.1186/1475-2859-13-66. View

18.

Lopez-Lopez A, Rogel M, Ormeno-Orrillo E, Martinez-Romero J, Martinez-Romero E . Phaseolus vulgaris seed-borne endophytic community with novel bacterial species such as Rhizobium endophyticum sp. nov. Syst Appl Microbiol. 2010; 33(6):322-7. DOI: 10.1016/j.syapm.2010.07.005. View

19.

Zhang Y, Wang E, Tian C, Wang F, Han L, Chen W . Bradyrhizobium elkanii, Bradyrhizobium yuanmingense and Bradyrhizobium japonicum are the main rhizobia associated with Vigna unguiculata and Vigna radiata in the subtropical region of China. FEMS Microbiol Lett. 2008; 285(2):146-54. DOI: 10.1111/j.1574-6968.2008.01169.x. View

20.

Broughton W, Dilworth M . Control of leghaemoglobin synthesis in snake beans. Biochem J. 1971; 125(4):1075-80. PMC: 1178271. DOI: 10.1042/bj1251075. View