Association of Androgenic Regulation and MicroRNAs in Acinar Adenocarcinoma of Prostate

Background: Prostate cancer represents 3.8% of cancer deaths worldwide. For most prostate cancer cells to grow, androgens need to bind to a cellular protein called the androgen receptor (AR). This study aims to demonstrate the expression of five microRNAs (miRs) and its influence on the AR formation in patients from the northern region of Brazil.

Material And Methods: Eighty-four tissue samples were investigated, including nodular prostatic hyperplasia (NPH) and acinar prostatic adenocarcinoma (CaP). Five miRs (27a-3p, 124, 130a, 488-3p, and 506) were quantified using the TaqMan Real Time PCR method and AR was measured using Western blotting.

Results: Levels of miRs 124, 130a, 488-3p, and 506 were higher in NPH samples. Conversely, in the CaP cases, higher levels of miR 27a-3p and AR were observed.

Conclusion: In the future, these microRNAs may be tested as markers of CaP at the serum level. The relative expression of AR was 20% higher in patients with prostate cancer, which suggests its potential as a biomarker for prostate malignancy.

Citing Articles

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Lu D, Shang J, Guo X, Zhang Y Comput Math Methods Med. 2022; 2022:7543619.

PMID: 36330452 PMC: 9626213. DOI: 10.1155/2022/7543619.

References

Fletcher C, Sulpice E, Combe S, Shibakawa A, Leach D, Hamilton M . Androgen receptor-modulatory microRNAs provide insight into therapy resistance and therapeutic targets in advanced prostate cancer. Oncogene. 2019; 38(28):5700-5724. PMC: 6755970. DOI: 10.1038/s41388-019-0823-5. View

Ramalho-Carvalho J, Martins J, Cekaite L, Sveen A, Torres-Ferreira J, Graca I . Epigenetic disruption of miR-130a promotes prostate cancer by targeting SEC23B and DEPDC1. Cancer Lett. 2016; 385:150-159. DOI: 10.1016/j.canlet.2016.10.028. View

Zhang T, Karsh L, Nissenblatt M, Canfield S . Androgen Receptor Splice Variant, AR-V7, as a Biomarker of Resistance to Androgen Axis-Targeted Therapies in Advanced Prostate Cancer. Clin Genitourin Cancer. 2019; 18(1):1-10. DOI: 10.1016/j.clgc.2019.09.015. View

Balacescu O, Dumitrescu R, Marian C . MicroRNAs Role in Prostate Cancer. Methods Mol Biol. 2018; 1856:103-117. DOI: 10.1007/978-1-4939-8751-1_6. View

Boll K, Reiche K, Kasack K, Morbt N, Kretzschmar A, Tomm J . MiR-130a, miR-203 and miR-205 jointly repress key oncogenic pathways and are downregulated in prostate carcinoma. Oncogene. 2012; 32(3):277-85. DOI: 10.1038/onc.2012.55. View

Fujita Y, Kojima T, Kawakami K, Mizutani K, Kato T, Deguchi T . miR-130a activates apoptotic signaling through activation of caspase-8 in taxane-resistant prostate cancer cells. Prostate. 2015; 75(14):1568-78. DOI: 10.1002/pros.23031. View

Conteduca V, Gurioli G, Brighi N, Lolli C, Schepisi G, Casadei C . Plasma Androgen Receptor in Prostate Cancer. Cancers (Basel). 2019; 11(11). PMC: 6896184. DOI: 10.3390/cancers11111719. View

Recouvreux M, Wu J, Gao A, Zonis S, Chesnokova V, Bhowmick N . Androgen Receptor Regulation of Local Growth Hormone in Prostate Cancer Cells. Endocrinology. 2017; 158(7):2255-2268. PMC: 5505214. DOI: 10.1210/en.2016-1939. View

Wang G, Zhao D, Spring D, DePinho R . Genetics and biology of prostate cancer. Genes Dev. 2018; 32(17-18):1105-1140. PMC: 6120714. DOI: 10.1101/gad.315739.118. View

10.

Bidarra D, Constancio V, Barros-Silva D, Ramalho-Carvalho J, Moreira-Barbosa C, Antunes L . Circulating MicroRNAs as Biomarkers for Prostate Cancer Detection and Metastasis Development Prediction. Front Oncol. 2019; 9:900. PMC: 6749029. DOI: 10.3389/fonc.2019.00900. View

11.

Altschuler J, Stockert J, Kyprianou N . Non-Coding RNAs Set a New Phenotypic Frontier in Prostate Cancer Metastasis and Resistance. Int J Mol Sci. 2021; 22(4). PMC: 7924036. DOI: 10.3390/ijms22042100. View

12.

Wu Z, Huang W, Chen B, Bai P, Wang X, Xing J . Up-regulation of miR-124 inhibits invasion and proliferation of prostate cancer cells through mediating JAK-STAT3 signaling pathway. Eur Rev Med Pharmacol Sci. 2017; 21(10):2338-2345. View

13.

Chua F, Adams B . Androgen receptor and miR-206 regulation in prostate cancer. Transcription. 2017; 8(5):313-327. PMC: 5703236. DOI: 10.1080/21541264.2017.1322668. View

14.

Zhang W, Mao Y, Wang H, Yin W, Zhu S, Wang W . MiR-124 suppresses cell motility and adhesion by targeting talin 1 in prostate cancer cells. Cancer Cell Int. 2015; 15:49. PMC: 4427999. DOI: 10.1186/s12935-015-0189-x. View

15.

Yan X, Yu H, Liu Y, Hou J, Yang Q, Zhao Y . miR-27a-3p Functions as a Tumor Suppressor and Regulates Non-Small Cell Lung Cancer Cell Proliferation via Targeting HOXB8. Technol Cancer Res Treat. 2019; 18:1533033819861971. PMC: 6640059. DOI: 10.1177/1533033819861971. View

16.

Wang W, Liu L, Li G, Chen Y, Li C, Jin D . Combined serum CA19-9 and miR-27a-3p in peripheral blood mononuclear cells to diagnose pancreatic cancer. Cancer Prev Res (Phila). 2013; 6(4):331-8. DOI: 10.1158/1940-6207.CAPR-12-0307. View

17.

Leal M, Ribeiro H, Rey J, Pinto G, Smith M, Moreira-Nunes C . YWHAE silencing induces cell proliferation, invasion and migration through the up-regulation of CDC25B and MYC in gastric cancer cells: new insights about YWHAE role in the tumor development and metastasis process. Oncotarget. 2016; 7(51):85393-85410. PMC: 5356744. DOI: 10.18632/oncotarget.13381. View

18.

Lyu J, Zhao L, Wang F, Ji J, Cao Z, Xu H . Discovery and Validation of Serum MicroRNAs as Early Diagnostic Biomarkers for Prostate Cancer in Chinese Population. Biomed Res Int. 2019; 2019:9306803. PMC: 6732591. DOI: 10.1155/2019/9306803. View

19.

Leal M, Mazzotti T, Calcagno D, Cirilo P, Martinez M, Demachki S . Deregulated expression of Nucleophosmin 1 in gastric cancer and its clinicopathological implications. BMC Gastroenterol. 2014; 14:9. PMC: 3893589. DOI: 10.1186/1471-230X-14-9. View

20.

Pena S, Di Pietro G, Fuchshuber-Moraes M, Genro J, Hutz M, Kehdy F . The genomic ancestry of individuals from different geographical regions of Brazil is more uniform than expected. PLoS One. 2011; 6(2):e17063. PMC: 3040205. DOI: 10.1371/journal.pone.0017063. View