Dihydromyricetin Enhances Intestinal Antioxidant Capacity of Growing-Finishing Pigs by Activating ERK/Nrf2/HO-1 Signaling Pathway

Overview

Journal Antioxidants (Basel)

Date 2022 Apr 23

PMID 35453388

Authors

Chuan Wei

Xiaoling Chen

Daiwen Chen

Bing Yu

Ping Zheng

Jun He

Hong Chen

Hui Yan

Yuheng Luo

Zhiqing Huang

Affiliations

Soon will be listed here.

Abstract

Oxidative stress is one of the main factors affecting animal health and reducing performance. The small intestine is the primary site of free-radical attacks. Dihydromyricetin (DHM) is a flavonoid compound with antioxidant, anti-inflammatory, and other biological activities, which is mainly extracted from Rattan tea. However, the effects of DHM on the intestinal antioxidant function of growing-finishing pigs and related mechanisms remain unclear. The aim of this study was to investigate the effect of dietary DHM supplementation on the intestinal antioxidant capacity of growing-finishing pigs and its mechanism. Our results show that dietary 0.03% DHM increased the activities of the total antioxidant capacity (T-AOC), catalase (CAT), and glutathione peroxidase (GSH-Px), decreased malondialdehyde (MDA) level, and upregulated protein expressions of HO-1, NQO1, nuclear Nrf2, and phospho-ERK (p-ERK) in the jejunum of growing-finishing pigs. Again, we found that 20 μmol/mL and 40 μmol/mL DHM treatment significantly upregulated the protein expression of HO-1 and promoted the nuclear translocation of Nrf2 and ERK phosphorylation in IPCE-J2 cells. ERK inhibitor PD98059 eliminated the DHM-induced upregulation of p-ERK, nuclear Nrf2, and HO-1. Our findings provided the first evidence that DHM enhanced the intestinal antioxidant capacity of growing-finishing pigs by activating the ERK/Nrf2/HO-1 signaling pathway.

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References

Giudice A, Montella M . Activation of the Nrf2-ARE signaling pathway: a promising strategy in cancer prevention. Bioessays. 2006; 28(2):169-81. DOI: 10.1002/bies.20359. View

Qi S, Xin Y, Guo Y, Diao Y, Kou X, Luo L . Ampelopsin reduces endotoxic inflammation via repressing ROS-mediated activation of PI3K/Akt/NF-κB signaling pathways. Int Immunopharmacol. 2011; 12(1):278-87. DOI: 10.1016/j.intimp.2011.12.001. View

Cao S, Shen Z, Wang C, Zhang Q, Hong Q, He Y . Resveratrol improves intestinal barrier function, alleviates mitochondrial dysfunction and induces mitophagy in diquat challenged piglets. Food Funct. 2019; 10(1):344-354. DOI: 10.1039/c8fo02091d. View

Jarmi T, Agarwal A . Heme oxygenase and renal disease. Curr Hypertens Rep. 2009; 11(1):56-62. DOI: 10.1007/s11906-009-0011-z. View

Chang Y, Yuan L, Liu J, Muhammad I, Cao C, Shi C . Dihydromyricetin attenuates Escherichia coli lipopolysaccharide-induced ileum injury in chickens by inhibiting NLRP3 inflammasome and TLR4/NF-κB signalling pathway. Vet Res. 2020; 51(1):72. PMC: 7247275. DOI: 10.1186/s13567-020-00796-8. View

Cho J, Kim Y, Choi B, Keum Y . Sulforaphane inhibition of TPA-mediated PDCD4 downregulation contributes to suppression of c-Jun and induction of p21-dependent Nrf2 expression. Eur J Pharmacol. 2014; 741:247-53. DOI: 10.1016/j.ejphar.2014.08.007. View

Xu X, Chen X, Chen D, Yu B, Yin J, Huang Z . Effects of dietary apple polyphenol supplementation on carcass traits, meat quality, muscle amino acid and fatty acid composition in finishing pigs. Food Funct. 2019; 10(11):7426-7434. DOI: 10.1039/c9fo01304k. View

Song Z, Tong G, Xiao K, Jiao L, Ke Y, Hu C . L-cysteine protects intestinal integrity, attenuates intestinal inflammation and oxidant stress, and modulates NF-κB and Nrf2 pathways in weaned piglets after LPS challenge. Innate Immun. 2016; 22(3):152-61. DOI: 10.1177/1753425916632303. View

Boler D, Fernandez-Duenas D, Kutzler L, Zhao J, Harrell R, Campion D . Effects of oxidized corn oil and a synthetic antioxidant blend on performance, oxidative status of tissues, and fresh meat quality in finishing barrows. J Anim Sci. 2012; 90(13):5159-69. DOI: 10.2527/jas.2012-5266. View

10.

Canella R, Benedusi M, Martini M, Cervellati F, Cavicchio C, Valacchi G . Role of Nrf2 in preventing oxidative stress induced chloride current alteration in human lung cells. J Cell Physiol. 2017; 233(8):6018-6027. DOI: 10.1002/jcp.26416. View

11.

Bagchi D, Carryl O, Tran M, Bagchi M, Garg A, Milnes M . Acute and chronic stress-induced oxidative gastrointestinal mucosal injury in rats and protection by bismuth subsalicylate. Mol Cell Biochem. 1999; 196(1-2):109-16. View

12.

Zhu L, Zhao K, Chen X, Xu J . Impact of weaning and an antioxidant blend on intestinal barrier function and antioxidant status in pigs. J Anim Sci. 2012; 90(8):2581-9. DOI: 10.2527/jas.2012-4444. View

13.

Niu Y, He J, Ahmad H, Shen M, Zhao Y, Gan Z . Dietary Curcumin Supplementation Increases Antioxidant Capacity, Upregulates Nrf2 and Hmox1 Levels in the Liver of Piglet Model with Intrauterine Growth Retardation. Nutrients. 2019; 11(12). PMC: 6950043. DOI: 10.3390/nu11122978. View

14.

Wolter B, Ellis M, DeDecker J, Curtis S, Hollis G, Shanks R . Effects of double stocking and weighing frequency on pig performance in wean-to-finish production systems. J Anim Sci. 2002; 80(6):1442-50. DOI: 10.2527/2002.8061442x. View

15.

Wolter B, Ellis M, Corrigan B, DeDecker J, Curtis S, Parr E . Impact of early postweaning growth rate as affected by diet complexity and space allocation on subsequent growth performance of pigs in a wean-to-finish production system. J Anim Sci. 2003; 81(2):353-9. DOI: 10.2527/2003.812353x. View

16.

Van Le Thanh B, Lemay M, Bastien A, Lapointe J, Lessard M, Chorfi Y . The potential effects of antioxidant feed additives in mitigating the adverse effects of corn naturally contaminated with Fusarium mycotoxins on antioxidant systems in the intestinal mucosa, plasma, and liver in weaned pigs. Mycotoxin Res. 2016; 32(2):99-116. DOI: 10.1007/s12550-016-0245-y. View

17.

Naito Y, Takagi T, Yoshikawa T . Heme oxygenase-1: a new therapeutic target for inflammatory bowel disease. Aliment Pharmacol Ther. 2004; 20 Suppl 1:177-84. DOI: 10.1111/j.1365-2036.2004.01992.x. View

18.

Son Y, Cheong Y, Kim N, Chung H, Kang D, Pae H . Mitogen-Activated Protein Kinases and Reactive Oxygen Species: How Can ROS Activate MAPK Pathways?. J Signal Transduct. 2011; 2011:792639. PMC: 3100083. DOI: 10.1155/2011/792639. View

19.

Silva-Guillen Y, Arellano C, Boyd R, Martinez G, van Heugten E . Growth performance, oxidative stress and immune status of newly weaned pigs fed peroxidized lipids with or without supplemental vitamin E or polyphenols. J Anim Sci Biotechnol. 2020; 11:22. PMC: 7057648. DOI: 10.1186/s40104-020-0431-9. View

20.

Johnson G, Lapadat R . Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science. 2002; 298(5600):1911-2. DOI: 10.1126/science.1072682. View