Antimicrobial Resistance and Molecular Characterization of Recovered from Cows with Clinical Mastitis in Dairy Herds from Southeastern Brazil

Overview

Journal Antibiotics (Basel)

Specialty Pharmacology

Date 2022 Apr 23

PMID 35453176

Authors

Gustavo Freu

Tiago Tomazi

Antonio F S Filho

Marcos B Heinemann

Marcos V Dos Santos

Affiliations

Soon will be listed here.

Abstract

is a contagious pathogen frequently associated with bovine mastitis in Brazil. Molecular characterization of isolated from affected mammary quarters of cows with clinical mastitis (CM) can provide data on epidemiological behavior of this pathogen and antimicrobial susceptibility (AMS) assessment at the genotypic level. This study genotypically characterized isolates recovered from cows with CM and determined the association of genotypes and AMS. A total of 84 strains identified from affected mammary quarters of cows with CM in 13 dairy herds from Southeastern Brazil were submitted for susceptibility testing to 10 antimicrobials using the technique of minimal inhibitory concentration. The same isolates were also genotyped using the spa-typing methodology. Results showed a high genotypic similarity between the isolates within and between herds, which were categorized as resistant to most antimicrobials, especially to β-lactam antibiotics. In addition, differences in AMS were observed among genotypic clusters, which may affect the efficacy of antimicrobials used to treat CM in different dairy herds.

Citing Articles

Biofilm Formation and Antimicrobial Resistance of and Isolates from Bovine Mastitis.

Fidelis C, Orsi A, Freu G, Goncalves J, Dos Santos M Vet Sci. 2024; 11(4).

PMID: 38668437 PMC: 11053524. DOI: 10.3390/vetsci11040170.

Quarter-wise proportion and beta-lactam resistance rate of bovine mastitis associated- among infectious episodes in Ethiopia: Systematic review and meta-Analysis.

Fenta M, Tafere F, Mebratu A, Malede B Heliyon. 2023; 9(7):e18180.

PMID: 37519650 PMC: 10372662. DOI: 10.1016/j.heliyon.2023.e18180.

Current Limitations of Staph Infection Diagnostics, and the Role for VOCs in Achieving Culture-Independent Detection.

Jenkins C, Bean H Pathogens. 2023; 12(2).

PMID: 36839453 PMC: 9963134. DOI: 10.3390/pathogens12020181.

Prevalence of staphylococcal toxin in food contaminated by Staphylococcus spp.: Protocol for a systematic review with meta-analysis.

Freitas J, de Assis C, Oliveira T, Maia C, de Sousa B, Medeiros G PLoS One. 2023; 18(2):e0282111.

PMID: 36809532 PMC: 9942949. DOI: 10.1371/journal.pone.0282111.

The prevalence of bovine mastitis-associated in China and its antimicrobial resistance rate: A meta-analysis.

Wang K, Cha J, Liu K, Deng J, Yang B, Xu H Front Vet Sci. 2022; 9:1006676.

PMID: 36439336 PMC: 9687384. DOI: 10.3389/fvets.2022.1006676.

References

Harmsen D, Claus H, Witte W, Rothganger J, Claus H, Turnwald D . Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J Clin Microbiol. 2003; 41(12):5442-8. PMC: 309029. DOI: 10.1128/JCM.41.12.5442-5448.2003. View

Rossi B, Bonsaglia E, Castilho I, Dantas S, Salina A, Langoni H . Genotyping of long term persistent Staphylococcus aureus in bovine subclinical mastitis. Microb Pathog. 2019; 132:45-50. DOI: 10.1016/j.micpath.2019.04.031. View

Guo Y, Song G, Sun M, Wang J, Wang Y . Prevalence and Therapies of Antibiotic-Resistance in . Front Cell Infect Microbiol. 2020; 10:107. PMC: 7089872. DOI: 10.3389/fcimb.2020.00107. View

Dittmann K, Chaul L, Lee S, Corassin C, Fernandes de Oliveira C, Pereira De Martinis E . in Some Brazilian Dairy Industries: Changes of Contamination and Diversity. Front Microbiol. 2017; 8:2049. PMC: 5662873. DOI: 10.3389/fmicb.2017.02049. View

Li T, Lu H, Wang X, Gao Q, Dai Y, Shang J . Molecular Characteristics of Causing Bovine Mastitis between 2014 and 2015. Front Cell Infect Microbiol. 2017; 7:127. PMC: 5395632. DOI: 10.3389/fcimb.2017.00127. View

Pedersen R, Kromker V, Bjarnsholt T, Dahl-Pedersen K, Buhl R, Jorgensen E . Biofilm Research in Bovine Mastitis. Front Vet Sci. 2021; 8:656810. PMC: 8138050. DOI: 10.3389/fvets.2021.656810. View

Sabat A, Budimir A, Nashev D, Sa-Leao R, van Dijl J, Laurent F . Overview of molecular typing methods for outbreak detection and epidemiological surveillance. Euro Surveill. 2013; 18(4):20380. DOI: 10.2807/ese.18.04.20380-en. View

Bonsaglia E, Silva N, Rossi B, Camargo C, Dantas S, Langoni H . Molecular epidemiology of methicillin-susceptible Staphylococcus aureus (MSSA) isolated from milk of cows with subclinical mastitis. Microb Pathog. 2018; 124:130-135. DOI: 10.1016/j.micpath.2018.08.031. View

Wang X, Wang Y, Guo G, Usman T, Hao D, Tang X . Antimicrobial resistance and toxin gene profiles of Staphylococcus aureus strains from Holstein milk. Lett Appl Microbiol. 2014; 58(6):527-34. DOI: 10.1111/lam.12221. View

10.

Haveri M, Roslof A, Rantala L, Pyorala S . Virulence genes of bovine Staphylococcus aureus from persistent and nonpersistent intramammary infections with different clinical characteristics. J Appl Microbiol. 2007; 103(4):993-1000. DOI: 10.1111/j.1365-2672.2007.03356.x. View

11.

Ruegg P . A 100-Year Review: Mastitis detection, management, and prevention. J Dairy Sci. 2017; 100(12):10381-10397. DOI: 10.3168/jds.2017-13023. View

12.

Barkema H, Schukken Y, Zadoks R . Invited Review: The role of cow, pathogen, and treatment regimen in the therapeutic success of bovine Staphylococcus aureus mastitis. J Dairy Sci. 2006; 89(6):1877-95. DOI: 10.3168/jds.S0022-0302(06)72256-1. View

13.

Deyno S, Fekadu S, Astatkie A . Resistance of to antimicrobial agents in Ethiopia: a meta-analysis. Antimicrob Resist Infect Control. 2017; 6:85. PMC: 5569497. DOI: 10.1186/s13756-017-0243-7. View

14.

Pu W, Su Y, Li J, Li C, Yang Z, Deng H . High incidence of oxacillin-susceptible mecA-positive Staphylococcus aureus (OS-MRSA) associated with bovine mastitis in China. PLoS One. 2014; 9(2):e88134. PMC: 3921137. DOI: 10.1371/journal.pone.0088134. View

15.

Vaughn J, Abdi R, Gillespie B, Kerro Dego O . Genetic diversity and virulence characteristics of Staphylococcus aureus isolates from cases of bovine mastitis. Microb Pathog. 2020; 144:104171. DOI: 10.1016/j.micpath.2020.104171. View

16.

Tomazi T, Ferreira G, Orsi A, Goncalves J, Ospina P, Nydam D . Association of herd-level risk factors and incidence rate of clinical mastitis in 20 Brazilian dairy herds. Prev Vet Med. 2018; 161:9-18. DOI: 10.1016/j.prevetmed.2018.10.007. View

17.

Middleton J, Fox L, Gay J, Tyler J, Besser T . Use of pulsed-field gel electrophoresis for detecting differences in Staphylococcus aureus strain populations between dairy herds with different cattle importation practices. Epidemiol Infect. 2002; 129(2):387-95. PMC: 2869898. DOI: 10.1017/s095026880200746x. View

18.

Mora-Hernandez Y, Vera Murguia E, Stinenbosch J, Hernandez Jauregui P, van Dijl J, Buist G . Molecular typing and antimicrobial resistance profiling of 33 mastitis-related Staphylococcus aureus isolates from cows in the Comarca Lagunera region of Mexico. Sci Rep. 2021; 11(1):6912. PMC: 7994548. DOI: 10.1038/s41598-021-86453-2. View

19.

Oliveira L, Hulland C, Ruegg P . Characterization of clinical mastitis occurring in cows on 50 large dairy herds in Wisconsin. J Dairy Sci. 2013; 96(12):7538-49. DOI: 10.3168/jds.2012-6078. View

20.

da Silva Abreu A, Matos L, da Silva Candido T, Barboza G, de Souza V, Nunez K . Antimicrobial resistance of Staphylococcus spp. isolated from organic and conventional Minas Frescal cheese producers in São Paulo, Brazil. J Dairy Sci. 2021; 104(4):4012-4022. DOI: 10.3168/jds.2020-19338. View