Assessing Potential Pathogenicity of Novel Highly Pathogenic Avian Influenza (H5N6) Viruses Isolated from Mongolian Wild Duck Feces Using a Mouse Model

Overview

Journal Emerg Microbes Infect

Specialty Infectious Diseases

Date 2022 Apr 22

PMID 35451353

Authors

Bao Tuan Duong

Duc Duong Than

Ulaankhuu Ankhanbaatar

Delgerzul Gombo-Ochir

Gansukh Shura

Amartuvshin Tsolmon

Chris Ka Pun Mok

Ganzorig Basan

Seon Ju Yeo

Hyun Park

Affiliations

Soon will be listed here.

Abstract

Several novel highly pathogenic avian influenza (HPAIVs) A(H5N6) viruses were reported in Mongolia in 2020, some of which included host-specific markers associated with mammalian infection. However, their pathogenicity has not yet been investigated. Here, we isolated and evaluate two novel genotypes of A(H5N6) subtype in Mongolia during 2018-2019 (A/wildDuck/MN/H5N6/2018-19). Their evolution pattern and molecular characteristics were evaluated using gene sequencing and their pathogenicity was determined using a mouse model. We also compared their antigenicity with previous H5 Clade 2.3.4.4 human isolates by cross-hemagglutination inhibition (HI). Our data suggests that A/wildDuck/MN/H5N6/2018-19 belongs to clade 2.3.4.4h, and maintains several residues associated with mammal adaptation. In addition, our evaluations revealed that their isolates are less virulent in mice than the previously identified H5 human isolates. However, their antigenicity is distinct from other HPAIVs H5 clade 2.3.4.4, thus supporting their continued evaluation as potential infection risks and the preparation of novel candidate vaccines for their neutralization.

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