C/EBPα Regulates 1 and 2 Trehalase-Related Bt Resistance in (L.)

Overview

Journal Insects

Specialty Biology

Date 2022 Apr 21

PMID 35447782

Authors

Jia Liu

Zheming Liu

Haihao Ma

Yaying Yu

Chengjia Zhang

Wei Zheng

Yilong Man

Hang Zhu

Yong Zhou

Xi Chen

Xiaomao Zhou

Aiping Zeng

Affiliations

Soon will be listed here.

Abstract

Trehalase regulates energy metabolism in insects by converting trehalose into two glucose molecules. High amounts of trehalase are critical for insect flight and larval stress resistance. However, whether trehalase participates in the development of pesticide resistance remains unclear. In this study, we explored this phenomenon and the mechanism that underlies the regulation of transcription. We found that overexpression of 1 and 2 induced (Bt) resistance in . The promoter sequences of 1 and 2 were also cloned and identified. The dual-luciferase reporter system and RNA interference technology revealed that the expression of 1 and 2 genes is possibly regulated by the CCAAT enhancer-binding protein (C/EBPα). A yeast one-hybrid experiment confirmed the interaction between C/EBPα and the 2 promoter. The findings of this study suggest that C/EBPα mediates the adaptability of to adverse environmental stressors by regulating the expression of trehalase.

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PMID: 36361800 PMC: 9656409. DOI: 10.3390/ijms232113005.

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