Low-Dose Gamma Radiation Modulates Liver and Testis Tissues Response to Acute Whole Body Irradiation

Overview

Journal Dose Response

Publisher Sage Publications

Date 2022 Apr 21

PMID 35444513

Authors

Nahed Abdel-Aziz

Riham A-H Haroun

Hebatallah E Mohamed

Affiliations

Soon will be listed here.

Abstract

Aim: This work aims to investigate whether the pre-exposure to low dose/low dose rate (40 mGy, 2.2 mGy/hour) γ-radiation as a priming dose can produce a protective effect against the subsequent high one (4 Gy, .425 Gy/minute).

Methods: Rats were divided into Group I (control), Group II (L); exposed to 40 mGy, Group III (H); exposed to 4 Gy, and Group IV (L+H); exposed to 40 mGy 24 hours before the exposure to 4Gy. The molecular and biochemical changes related to oxidative stress, DNA damage, apoptosis, and mitochondrial activity in the liver and testis were studied 4 hours after irradiation.

Results: Exposure to 40 mGy before 4 Gy induced a significant increase in the levels of Nrf2, Nrf2 mRNA, TAC, and mitochondrial complexes I & II accompanied by a significant decrease in the levels of LPO, 8-OHdG, DNA fragmentation, TNF-α, caspase-3, and caspase-3 mRNA compared with H group.

Conclusion: Exposure to low-dose γ-radiation before a high dose provides protective mechanisms that allow the body to survive better after exposure to a subsequent high one via reducing the oxidative stress, DNA damage, and apoptosis-induced early after irradiation. However, further studies are required to identify the long-term effects of this low dose.

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References

Lopez-Nieva P, Malave M, Gonzalez-Sanchez L, Fernandez-Piqueras J, Fernandez-Navarro P, Santos J . Transcriptomic analysis reveals sex-specific differences in the expression of Dcl1 and Fis1 genes in the radio-adaptive response of thymocytes to TRP53-mediated apoptosis. BMC Genomics. 2016; 17:698. PMC: 5007679. DOI: 10.1186/s12864-016-3036-0. View

Wang B, Ohyama H, Shang Y, Tanaka K, Aizawa S, Yukawa O . Adaptive response in embryogenesis: V. Existence of two efficient dose-rate ranges for 0.3 Gy of priming irradiation to adapt mouse fetuses. Radiat Res. 2004; 161(3):264-72. DOI: 10.1667/rr3141. View

Abd-ElRaouf A, Nada A, Mohammed N, Amer H, Abd-ElRahman S, Abdelsalam R . Low dose gamma irradiation attenuates cyclophosphamide-induced cardiotoxicity in rats: role of NF-κB signaling pathway. Int J Radiat Biol. 2021; 97(5):632-641. DOI: 10.1080/09553002.2021.1893856. View

Vaiserman A, Koliada A, Zabuga O, Socol Y . Health Impacts of Low-Dose Ionizing Radiation: Current Scientific Debates and Regulatory Issues. Dose Response. 2018; 16(3):1559325818796331. PMC: 6149023. DOI: 10.1177/1559325818796331. View

Feinendegen L . Quantification of Adaptive Protection Following Low-dose Irradiation. Health Phys. 2016; 110(3):276-80. DOI: 10.1097/HP.0000000000000431. View

Koturbash I, Merrifield M, Kovalchuk O . Fractionated exposure to low doses of ionizing radiation results in accumulation of DNA damage in mouse spleen tissue and activation of apoptosis in a p53/Atm-independent manner. Int J Radiat Biol. 2016; 93(2):148-155. PMC: 6709590. DOI: 10.1080/09553002.2017.1231943. View

Rithidech K, Udomtanakunchai C, Honikel L, Whorton E . No Evidence for the In Vivo Induction of Genomic Instability by Low Doses of CS Gamma Rays in Bone Marrow Cells of BALB/CJ and C57BL/6J Mice. Dose Response. 2012; 10(1):11-36. PMC: 3299525. DOI: 10.2203/dose-response.11-002.Rithidech. View

Abdelrazzak A, El-Missiry M, Ahmed M, Elnady B . Effect of low-dose X-rays on the liver of whole-body irradiated rats. Int J Radiat Biol. 2018; 95(3):264-273. DOI: 10.1080/09553002.2019.1554925. View

Agathokleous E, Kitao M, Calabrese E . Environmental hormesis and its fundamental biological basis: Rewriting the history of toxicology. Environ Res. 2018; 165:274-278. DOI: 10.1016/j.envres.2018.04.034. View

10.

Yonezawa M, Misonoh J, Hosokawa Y . Two types of X-ray-induced radioresistance in mice: presence of 4 dose ranges with distinct biological effects. Mutat Res. 1996; 358(2):237-43. DOI: 10.1016/s0027-5107(96)00126-1. View

11.

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

12.

Ibrahim D, Radwan R, Abdel Fattah S . Antioxidant and antiapoptotic effects of sea cucumber and valsartan against doxorubicin-induced cardiotoxicity in rats: The role of low dose gamma irradiation. J Photochem Photobiol B. 2017; 170:70-78. DOI: 10.1016/j.jphotobiol.2017.03.022. View

13.

Abdel-Aziz N, Elkady A, Elgazzar E . Effect of Low-Dose Gamma Radiation and Lipoic Acid on High- Radiation-Dose Induced Rat Brain Injuries. Dose Response. 2021; 19(4):15593258211044845. PMC: 8573698. DOI: 10.1177/15593258211044845. View

14.

Bakshi M, Azimzadeh O, Barjaktarovic Z, Kempf S, Merl-Pham J, Hauck S . Total body exposure to low-dose ionizing radiation induces long-term alterations to the liver proteome of neonatally exposed mice. J Proteome Res. 2014; 14(1):366-73. DOI: 10.1021/pr500890n. View

15.

WILLS E . Mechanisms of lipid peroxide formation in animal tissues. Biochem J. 1966; 99(3):667-76. PMC: 1265056. DOI: 10.1042/bj0990667. View

16.

Ji K, Wang Y, Du L, Xu C, Liu Y, He N . Research Progress on the Biological Effects of Low-Dose Radiation in China. Dose Response. 2019; 17(1):1559325819833488. PMC: 6393828. DOI: 10.1177/1559325819833488. View

17.

Marzban M, Anjamshoa M, Jafari P, Masoumi H, Ahadi R, Fatehi D . Effects of gamma rays on rat testis tissue according to the morphological parameters and immunohistochemistry: radioprotective role of silymarin. Electron Physician. 2017; 9(6):4524-4532. PMC: 5557131. DOI: 10.19082/4524. View

18.

Kostyuk S, Proskurnina E, Konkova M, Abramova M, Kalianov A, Ershova E . Effect of Low-Dose Ionizing Radiation on the Expression of Mitochondria-Related Genes in Human Mesenchymal Stem Cells. Int J Mol Sci. 2022; 23(1). PMC: 8745621. DOI: 10.3390/ijms23010261. View

19.

Sharma S, Singla N, Chadha V, Dhawan D . A concept of radiation hormesis: stimulation of antioxidant machinery in rats by low dose ionizing radiation. Hell J Nucl Med. 2019; 22(1):43-48. DOI: 10.1967/s002449910958. View

20.

Shin E, Lee S, Kang H, Kim J, Kim K, Youn H . Organ-Specific Effects of Low Dose Radiation Exposure: A Comprehensive Review. Front Genet. 2020; 11:566244. PMC: 7565684. DOI: 10.3389/fgene.2020.566244. View