Notch Controls the Cell Cycle to Define Leader Versus Follower Identities During Collective Cell Migration

Overview

Journal Elife

Specialty Biology

Date 2022 Apr 19

PMID 35438077

Authors

Zain Alhashem

Dylan Feldner-Busztin

Christopher Revell

Macarena Alvarez-Garcillan Portillo

Karen Camargo-Sosa

Joanna Richardson

Manuel Rocha

Anton Gauert

Tatianna Corbeaux

Martina Milanetto

Francesco Argenton

Natascia Tiso

Robert N Kelsh

Victoria E Prince

Katie Bentley

Claudia Linker

Affiliations

Soon will be listed here.

Abstract

Coordination of cell proliferation and migration is fundamental for life, and its dysregulation has catastrophic consequences, such as cancer. How cell cycle progression affects migration, and vice versa, remains largely unknown. We address these questions by combining in silico modelling and in vivo experimentation in the zebrafish trunk neural crest (TNC). TNC migrate collectively, forming chains with a leader cell directing the movement of trailing followers. We show that the acquisition of migratory identity is autonomously controlled by Notch signalling in TNC. High Notch activity defines leaders, while low Notch determines followers. Moreover, cell cycle progression is required for TNC migration and is regulated by Notch. Cells with low Notch activity stay longer in G and become followers, while leaders with high Notch activity quickly undergo G/S transition and remain in S-phase longer. In conclusion, TNC migratory identities are defined through the interaction of Notch signalling and cell cycle progression.

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