Functionalized Graphene Oxide in Microbial Engineering: An Effective Stimulator for Bacterial Growth

Overview

Journal Carbon N Y

Date 2022 Apr 18

PMID 35431318

Authors

Yinchan Luo

Xinxing Yang

Xiaofang Tan

Ligeng Xu

Zhuang Liu

Jie Xiao

Rui Peng

Affiliations

Soon will be listed here.

Abstract

Whether graphene and graphene oxide (GO) would affect the activities of bacteria has been under debate. Nevertheless, how graphene derivatives with biocompatible coatings interact with microorganisms and the underlying mechanisms are important issues for nanobiotechnology, and remain to be further explored. Herein, three new types of nano-GOs functionalized with polyethylene glycol (nGO-PEGs) were synthesized by varying the PEGylation degree, and their effects on were carefully investigated. Interestingly, nGO-PEG (1:1), the one with relatively lower PEGylation degree, could significantly stimulate bacterial growth, whereas as-made GO and the other two nGO-PEGs showed no effect. Further analysis revealed that nGO-PEG (1:1) treatment significantly accelerated FtsZ-ring assembly, shortening Phase 1 in the bacterial cell cycle. Both DNA synthesis and extracellular polymeric substance (EPS) secretion were also dramatically increased. This unique phenomenon suggests promising potentials in microbial engineering as well as in clinical detection of bacterial pathogens. As a proof-of-concept, nGO-PEG (1:1) treatment could remarkably enhance (up to 6-fold) recombinant protein production in engineered bacteria cells. To our best knowledge, this is the first demonstration of functionalized GO as a novel, positive regulator in microbial engineering. Moreover, our work highlights the critical role of surface chemistry in modulating the interactions between nanomaterials and microorganisms.

Citing Articles

Graphene-based nanomaterials for cancer therapy and anti-infections.

Wang Y, Li J, Li X, Shi J, Jiang Z, Zhang C Bioact Mater. 2022; 14:335-349.

PMID: 35386816 PMC: 8964986. DOI: 10.1016/j.bioactmat.2022.01.045.

Photothermal-assisted antibacterial application of graphene oxide-Ag nanocomposites against clinically isolated multi-drug resistant .

Chen Y, Wu W, Xu Z, Jiang C, Han S, Ruan J R Soc Open Sci. 2020; 7(7):192019.

PMID: 32874607 PMC: 7428222. DOI: 10.1098/rsos.192019.

Medium-Dependent Antibacterial Properties and Bacterial Filtration Ability of Reduced Graphene Oxide.

Gusev A, Zakharova O, Muratov D, Vorobeva N, Sarker M, Rybkin I Nanomaterials (Basel). 2019; 9(10).

PMID: 31614934 PMC: 6835404. DOI: 10.3390/nano9101454.

References

Liu X, Liu L, Wang Y, Wang X, Ma Y, Li Y . The Study on the factors affecting transformation efficiency of E. coli competent cells. Pak J Pharm Sci. 2014; 27(3 Suppl):679-84. View

Liu M, Song J, Shuang S, Dong C, Brennan J, Li Y . A graphene-based biosensing platform based on the release of DNA probes and rolling circle amplification. ACS Nano. 2014; 8(6):5564-73. DOI: 10.1021/nn5007418. View

Ma J, Drake P, Christou P . The production of recombinant pharmaceutical proteins in plants. Nat Rev Genet. 2003; 4(10):794-805. DOI: 10.1038/nrg1177. View

Zhang L, Lu Z, Zhao Q, Huang J, Shen H, Zhang Z . Enhanced chemotherapy efficacy by sequential delivery of siRNA and anticancer drugs using PEI-grafted graphene oxide. Small. 2011; 7(4):460-4. DOI: 10.1002/smll.201001522. View

Zhang L, Xia J, Zhao Q, Liu L, Zhang Z . Functional graphene oxide as a nanocarrier for controlled loading and targeted delivery of mixed anticancer drugs. Small. 2009; 6(4):537-44. DOI: 10.1002/smll.200901680. View

Dong H, Dong C, Ren T, Li Y, Shi D . Surface-engineered graphene-based nanomaterials for drug delivery. J Biomed Nanotechnol. 2015; 10(9):2086-106. DOI: 10.1166/jbn.2014.1989. View

Tu Y, Lv M, Xiu P, Huynh T, Zhang M, Castelli M . Destructive extraction of phospholipids from Escherichia coli membranes by graphene nanosheets. Nat Nanotechnol. 2013; 8(8):594-601. DOI: 10.1038/nnano.2013.125. View

Courcelle J, HANAWALT P . Participation of recombination proteins in rescue of arrested replication forks in UV-irradiated Escherichia coli need not involve recombination. Proc Natl Acad Sci U S A. 2001; 98(15):8196-202. PMC: 37421. DOI: 10.1073/pnas.121008898. View

Vincentelli R, Bignon C, Gruez A, Canaan S, Sulzenbacher G, Tegoni M . Medium-scale structural genomics: strategies for protein expression and crystallization. Acc Chem Res. 2003; 36(3):165-72. DOI: 10.1021/ar010130s. View

10.

Akhavan O, Ghaderi E, Esfandiar A . Wrapping bacteria by graphene nanosheets for isolation from environment, reactivation by sonication, and inactivation by near-infrared irradiation. J Phys Chem B. 2011; 115(19):6279-88. DOI: 10.1021/jp200686k. View

11.

Ang P, Li A, Jaiswal M, Wang Y, Hou H, Thong J . Flow sensing of single cell by graphene transistor in a microfluidic channel. Nano Lett. 2011; 11(12):5240-6. DOI: 10.1021/nl202579k. View

12.

Akhavan O, Ghaderi E . Toxicity of graphene and graphene oxide nanowalls against bacteria. ACS Nano. 2010; 4(10):5731-6. DOI: 10.1021/nn101390x. View

13.

Ferullo D, Cooper D, Moore H, Lovett S . Cell cycle synchronization of Escherichia coli using the stringent response, with fluorescence labeling assays for DNA content and replication. Methods. 2009; 48(1):8-13. PMC: 2746677. DOI: 10.1016/j.ymeth.2009.02.010. View

14.

Tian T, Shi X, Cheng L, Luo Y, Dong Z, Gong H . Graphene-based nanocomposite as an effective, multifunctional, and recyclable antibacterial agent. ACS Appl Mater Interfaces. 2014; 6(11):8542-8. DOI: 10.1021/am5022914. View

15.

Hong H, Yang K, Zhang Y, Engle J, Feng L, Yang Y . In vivo targeting and imaging of tumor vasculature with radiolabeled, antibody-conjugated nanographene. ACS Nano. 2012; 6(3):2361-70. PMC: 3314116. DOI: 10.1021/nn204625e. View

16.

Zhao Y, Ye C, Liu W, Chen R, Jiang X . Tuning the composition of AuPt bimetallic nanoparticles for antibacterial application. Angew Chem Int Ed Engl. 2014; 53(31):8127-31. PMC: 4320751. DOI: 10.1002/anie.201401035. View

17.

Zhu B, Niu Z, Wang H, Leow W, Wang H, Li Y . Microstructured graphene arrays for highly sensitive flexible tactile sensors. Small. 2014; 10(18):3625-31. DOI: 10.1002/smll.201401207. View

18.

Jin L, Yang K, Yao K, Zhang S, Tao H, Lee S . Functionalized graphene oxide in enzyme engineering: a selective modulator for enzyme activity and thermostability. ACS Nano. 2012; 6(6):4864-75. DOI: 10.1021/nn300217z. View

19.

Wang D, Wang G, Zhang G, Xu X, Yang F . Using graphene oxide to enhance the activity of anammox bacteria for nitrogen removal. Bioresour Technol. 2013; 131:527-30. DOI: 10.1016/j.biortech.2013.01.099. View

20.

Robinson J, Tabakman S, Liang Y, Wang H, Sanchez Casalongue H, Vinh D . Ultrasmall reduced graphene oxide with high near-infrared absorbance for photothermal therapy. J Am Chem Soc. 2011; 133(17):6825-31. DOI: 10.1021/ja2010175. View