Effect of Crystallinity and Related Surface Properties on Gene Expression of Primary Fibroblasts

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 Apr 15

PMID 35425452

Authors

Dorota Kolbuk

Marzena Ciechomska

Oliwia Jeznach

Pawel Sajkiewicz

Affiliations

Soon will be listed here.

Abstract

The biomaterial-cells interface is one of the most fundamental issues in tissue regeneration. Despite many years of scientific work, there is no clear answer to what determines the desired adhesion of cells and the synthesis of ECM proteins. Crystallinity is a characteristic of the structure that influences the surface and bulk properties of semicrystalline polymers used in medicine. The crystallinity of polycaprolactone (PCL) was varied by changing the molecular weight of the polymer and the annealing procedure. Measurements of surface free energy showed differences related to substrate crystallinity. Additionally, the water contact angle was determined to characterise surface wettability which was crucial in the analysis of protein absorption. X-ray photoelectron spectroscopy was used to indicate oxygen bonds amount on the surface. Finally, the impact of the crystallinity, and related properties were demonstrated on dermal fibroblasts' response. Cellular proliferation and expression of selected genes: α-SMA, collagen I, TIMP, integrin were analysed.

Citing Articles

On the Structural and Biological Effects of Hydroxyapatite and Gold Nano-Scale Particles in Poly(Vinylidene Fluoride) Smart Scaffolds for Bone and Neural Tissue Engineering.

Zaszczynska A, Zychowicz M, Kolbuk D, Denis P, Gradys A, Sajkiewicz P Molecules. 2025; 30(5).

PMID: 40076266 PMC: 11901919. DOI: 10.3390/molecules30051041.

Crystallinity of covalent organic frameworks controls immune responses.

Esrafili A, Thumsi A, Jaggarapu M, Nile R, Kupfer J, Dugoni M Nat Commun. 2024; 15(1):9739.

PMID: 39528477 PMC: 11555212. DOI: 10.1038/s41467-024-54227-9.

Development of Poly(methyl methacrylate)/nano-hydroxyapatite (PMMA/nHA) Nanofibers for Tissue Engineering Regeneration Using an Electrospinning Technique.

Zaszczynska A, Kolbuk D, Gradys A, Sajkiewicz P Polymers (Basel). 2024; 16(4).

PMID: 38399909 PMC: 10893281. DOI: 10.3390/polym16040531.

Biobran-loaded core/shell nanofibrous scaffold: a promising wound dressing candidate.

Kenawy E, El-Moaty M, Ghoneum M, Soliman H, El-Shanshory A, Shendy S RSC Adv. 2024; 14(7):4930-4945.

PMID: 38327812 PMC: 10848241. DOI: 10.1039/d3ra08609g.

Chitosan-Based High-Intensity Modification of the Biodegradable Substitutes for Cancellous Bone.

Kolakowska A, Kolbuk D, Chwojnowski A, Rafalski A, Gadomska-Gajadhur A J Funct Biomater. 2023; 14(8).

PMID: 37623655 PMC: 10455456. DOI: 10.3390/jfb14080410.

References

Ribeiro J, Forte T, Tavares S, Andrade F, Silveira Vieira R, Lima V . The effects of the molecular weight of chitosan on the tissue inflammatory response. J Biomed Mater Res A. 2021; 109(12):2556-2569. DOI: 10.1002/jbm.a.37250. View

Hinz B, Celetta G, Tomasek J, Gabbiani G, Chaponnier C . Alpha-smooth muscle actin expression upregulates fibroblast contractile activity. Mol Biol Cell. 2001; 12(9):2730-41. PMC: 59708. DOI: 10.1091/mbc.12.9.2730. View

Ng F, Ong Y, Chen H, Tran L, Cao Y, Tay B . A facile method for fabricating a three-dimensional aligned fibrous scaffold for vascular application. RSC Adv. 2022; 9(23):13054-13064. PMC: 9063778. DOI: 10.1039/c9ra00661c. View

Mahmoudi P, Matsen M . Entropic segregation of short polymers to the surface of a polydisperse melt. Eur Phys J E Soft Matter. 2017; 40(10):85. DOI: 10.1140/epje/i2017-11575-7. View

Kim C, Ye F, Ginsberg M . Regulation of integrin activation. Annu Rev Cell Dev Biol. 2011; 27:321-45. DOI: 10.1146/annurev-cellbio-100109-104104. View

Thevenot P, Hu W, Tang L . Surface chemistry influences implant biocompatibility. Curr Top Med Chem. 2008; 8(4):270-80. PMC: 3230929. DOI: 10.2174/156802608783790901. View

Mikos A, Sarakinos G, Lyman M, Ingber D, Vacanti J, Langer R . Prevascularization of porous biodegradable polymers. Biotechnol Bioeng. 1993; 42(6):716-23. DOI: 10.1002/bit.260420606. View

Hu W, Eaton J, Ugarova T, Tang L . Molecular basis of biomaterial-mediated foreign body reactions. Blood. 2001; 98(4):1231-8. DOI: 10.1182/blood.v98.4.1231. View

Liu F, Mih J, Shea B, Kho A, Sharif A, Tager A . Feedback amplification of fibrosis through matrix stiffening and COX-2 suppression. J Cell Biol. 2010; 190(4):693-706. PMC: 2928007. DOI: 10.1083/jcb.201004082. View

10.

Pakyari M, Farrokhi A, Khosravi Maharlooei M, Ghahary A . Critical Role of Transforming Growth Factor Beta in Different Phases of Wound Healing. Adv Wound Care (New Rochelle). 2014; 2(5):215-224. PMC: 3857353. DOI: 10.1089/wound.2012.0406. View

11.

Sanchez L, Brack N, Postma A, Pigram P, Meagher L . Surface modification of electrospun fibres for biomedical applications: A focus on radical polymerization methods. Biomaterials. 2016; 106:24-45. DOI: 10.1016/j.biomaterials.2016.08.011. View

12.

Jeznach O, Kolbuk D, Sajkiewicz P . Aminolysis of Various Aliphatic Polyesters in a Form of Nanofibers and Films. Polymers (Basel). 2019; 11(10). PMC: 6835534. DOI: 10.3390/polym11101669. View

13.

Ciechomska M, OReilly S, Suwara M, Bogunia-Kubik K, van Laar J . MiR-29a reduces TIMP-1 production by dermal fibroblasts via targeting TGF-β activated kinase 1 binding protein 1, implications for systemic sclerosis. PLoS One. 2014; 9(12):e115596. PMC: 4280195. DOI: 10.1371/journal.pone.0115596. View

14.

Wang K, Cai L, Jesse S, Wang S . Poly(ε-caprolactone)-banded spherulites and interaction with MC3T3-E1 cells. Langmuir. 2012; 28(9):4382-95. DOI: 10.1021/la205162d. View

15.

Smith M, Chalklen T, Lindackers C, Calahorra Y, Howe C, Tamboli A . Poly-l-Lactic Acid Nanotubes as Soft Piezoelectric Interfaces for Biology: Controlling Cell Attachment Polymer Crystallinity. ACS Appl Bio Mater. 2020; 3(4):2140-2149. PMC: 7175596. DOI: 10.1021/acsabm.0c00012. View

16.

Simon Jr C, Eidelman N, Kennedy S, Sehgal A, Khatri C, Washburn N . Combinatorial screening of cell proliferation on poly(L-lactic acid)/poly(D,L-lactic acid) blends. Biomaterials. 2005; 26(34):6906-15. DOI: 10.1016/j.biomaterials.2005.04.050. View

17.

Gorecka Z, Idaszek J, Kolbuk D, Choinska E, Chlanda A, Swieszkowski W . The effect of diameter of fibre on formation of hydrogen bonds and mechanical properties of 3D-printed PCL. Mater Sci Eng C Mater Biol Appl. 2020; 114:111072. DOI: 10.1016/j.msec.2020.111072. View

18.

Washburn N, Yamada K, Simon Jr C, Kennedy S, Amis E . High-throughput investigation of osteoblast response to polymer crystallinity: influence of nanometer-scale roughness on proliferation. Biomaterials. 2003; 25(7-8):1215-24. DOI: 10.1016/j.biomaterials.2003.08.043. View

19.

Innocenti M . New insights into the formation and the function of lamellipodia and ruffles in mesenchymal cell migration. Cell Adh Migr. 2018; 12(5):401-416. PMC: 6363039. DOI: 10.1080/19336918.2018.1448352. View

20.

Kim M, Carman C, Springer T . Bidirectional transmembrane signaling by cytoplasmic domain separation in integrins. Science. 2003; 301(5640):1720-5. DOI: 10.1126/science.1084174. View