Inhibitory Properties of Saponin from Peel Against α-glucosidase

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 Apr 15

PMID 35424054

Authors

Yipeng Gu

Xiaomei Yang

Chaojie Shang

Truong Thi Phuong Thao

Tomoyuki Koyama

Affiliations

Soon will be listed here.

Abstract

The inhibitory properties towards α-glucosidase and elevation of postprandial glycemia in mice by the saponin constituent from peel were evaluated for the first time. Three saponins were isolated by silica gel and HPLC, identified as stigmasterol glucoside, campesterol glucoside and daucosterol by NMR spectroscopy. Daucosterol presented the highest content and showed the strongest α-glucosidase inhibitory activity with competitive inhibition. Static fluorescence quenching of α-glucosidase was caused by the formation of the daucosterol-α-glucosidase complex, which was mainly derived from hydrogen bonds and van der Waals forces. Daucosterol formed 7 hydrogen bonds with 4 residues of the active site and produced hydrophobic interactions with 3 residues located at the exterior part of the binding pocket. The maltose-loading test results showed that daucosterol inhibited elevation of postprandial glycemia in ddY mice. This suggests that daucosterol from peel can potentially be used as a food supplement for anti-hyperglycemia.

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