Synthesis of the Pentasaccharide Repeating Unit from and Measurement of Its Inflammatory Properties

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 Apr 15

PMID 35424013

Authors

Teron Haynie

Shawn Gubler

Christoph Drees

Tanner Heaton

Tanner Mitton

Quinn Gleave

Albert Bendelac

Shenglou Deng

Paul B Savage

Affiliations

Soon will be listed here.

Abstract

The roles played by the gut microbiome in human health are increasingly recognized, and the prevalence of specific microorganisms has been correlated with different diseases. For example, blooms of the Gram-positive bacterium have been correlated with inflammatory bowel disease, and recently a polysaccharide produced by this organism was shown to stimulate release of inflammatory cytokines. This stimulation was proposed to signal through toll-like receptor 4 (TLR4). We have synthesized the pentasaccharide repeating unit of this polysaccharide and showed that it stimulates TNF-α and IL-6 release from bone marrow-derived dendritic cells (BMDCs) in a TLR4-dependent manner. A related glycan does not stimulate significant cytokine release, demonstrating TLR4 selectivity in glycan recognition.

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