The First Genome Assembly and Sex Marker Identification of Pluang Chomphu Fish () from Southern Thailand

The genus belongs to the group of cyprinid fish commonly known as mahseer. Although species are rapidly declining in the wild, and some face extinction, ambiguities in species identification hinder their collection and conservation. We conducted a genome survey of male and female collected in Thailand. The genome sizes of the male and female fish were approximately 1,671 and 1,645 Mb, respectively, with repeat contents of approximately 33%. The heterozygosity ratios of the male and female fish, which were 0.34% and 0.39%, respectively, suggested that the sex of is determined by the ZW system. A sex marker was identified and confirmed by PCR amplification. The result indicated that has a ZZ/ZW sex determination system. Subsequently, comparative genomic and phylogenetic analyses of and other fish in the Cyprinidae family were performed to explore the genetic diversity and evolution of the species. We also assembled the complete mitochondrial genome sequences of the collected in Thailand. A phylogenetic tree of different species, constructed based on mitochondrial genome sequences, indicated that was closely related to We believe this is the first genome survey of a species from the genus or Mahseer group. Our results may help identify species, providing a reference for genetic studies of the genus and other mahseer fish.