Molecular Cloning of Two Cysteine Proteinases from Paw-paw (Carica Papaya)

Overview

Journal Biochem J

Specialty Biochemistry

Date 1986 Jul 1

PMID 3541893

Citations 5

Authors

R A McKee

S Adams

J A Matthews

C J Smith

H Smith

Affiliations

Soon will be listed here.

Abstract

Two cDNA clones for plant cysteine proteinases have been isolated from a Carica papaya (paw-paw, papaya) leaf tissue cDNA library by using a mixture of 16 synthetic oligodeoxyribonucleotides as a hybridization probe. The inserted regions are 311 and 440 base-pairs in length and have the potential to encode a region corresponding to the C-terminal region of two proteins which are homologous with the known plant cysteine proteinases and the mammalian thiol cathepsins. One of the sequences shows a high (greater than 77%) homology with the plant cysteine proteinase papain, the other is closely related to papaya chymopapain. One sequence contains all, and the other most, of the 3' untranslated region of the mRNA. The inserts were used as specific probes in Northern Blot analyses giving an estimated size for the two mRNA species of 1.45 kilobases.

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References

KIRSCHKE H, Locnikar P, Turk V . Species variations amongst lysosomal cysteine proteinases. FEBS Lett. 1984; 174(1):123-7. DOI: 10.1016/0014-5793(84)81089-3. View

Buttle D, Barrett A . Chymopapain. Chromatographic purification and immunological characterization. Biochem J. 1984; 223(1):81-8. PMC: 1144267. DOI: 10.1042/bj2230081. View

Kamphuis I, Kalk K, Swarte M, Drenth J . Structure of papain refined at 1.65 A resolution. J Mol Biol. 1984; 179(2):233-56. DOI: 10.1016/0022-2836(84)90467-4. View

Ohno S, Emori Y, Imajoh S, Kawasaki H, Kisaragi M, Suzuki K . Evolutionary origin of a calcium-dependent protease by fusion of genes for a thiol protease and a calcium-binding protein?. Nature. 1984; 312(5994):566-70. DOI: 10.1038/312566a0. View

Gay N, Walker J . Molecular cloning of a bovine cathepsin. Biochem J. 1985; 225(3):707-12. PMC: 1144647. DOI: 10.1042/bj2250707. View

San Segundo B, Chan S, Steiner D . Identification of cDNA clones encoding a precursor of rat liver cathepsin B. Proc Natl Acad Sci U S A. 1985; 82(8):2320-4. PMC: 397549. DOI: 10.1073/pnas.82.8.2320. View

Brocklehurst K, Salih E, McKee R, Smith H . Fresh non-fruit latex of Carica papaya contains papain, multiple forms of chymopapain A and papaya proteinase omega. Biochem J. 1985; 228(2):525-7. PMC: 1145013. DOI: 10.1042/bj2280525. View

Barrett A, Buttle D . Names and numbers of papaya proteinases. Biochem J. 1985; 228(2):527. PMC: 1145014. DOI: 10.1042/bj2280527. View

Mitchel R, Chaiken I, Smith E . The complete amino acid sequence of papain. Additions and corrections. J Biol Chem. 1970; 245(14):3485-92. View

10.

Mandel M, Higa A . Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970; 53(1):159-62. DOI: 10.1016/0022-2836(70)90051-3. View

11.

Drenth J, Jansonius J, Koekoek R, Wolthers B . The structure of papain. Adv Protein Chem. 1971; 25:79-115. DOI: 10.1016/s0065-3233(08)60279-x. View

12.

Grunstein M, Hogness D . Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975; 72(10):3961-5. PMC: 433117. DOI: 10.1073/pnas.72.10.3961. View

13.

Goto K, Murachi T, Takahashi N . Structural studies on stem bromelain isolation, characterization and alignment of the cyanogen bromide fragments. FEBS Lett. 1976; 62(1):93-5. DOI: 10.1016/0014-5793(76)80024-5. View

14.

Bantle J, Maxwell I, Hahn W . Specificity of oligo (dT)-cellulose chromatography in the isolation of polyadenylated RNA. Anal Biochem. 1976; 72:413-27. DOI: 10.1016/0003-2697(76)90549-2. View

15.

Proudfoot N, Brownlee G . 3' non-coding region sequences in eukaryotic messenger RNA. Nature. 1976; 263(5574):211-4. DOI: 10.1038/263211a0. View

16.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

17.

Carne A, Moore C . The amino acid sequence of the tryptic peptides from actinidin, a proteolytic enzyme from the fruit of Actinidia chinensis. Biochem J. 1978; 173(1):73-83. PMC: 1185751. DOI: 10.1042/bj1730073. View

18.

Noyes B, Mevarech M, Stein R, Agarwal K . Detection and partial sequence analysis of gastrin mRNA by using an oligodeoxynucleotide probe. Proc Natl Acad Sci U S A. 1979; 76(4):1770-4. PMC: 383472. DOI: 10.1073/pnas.76.4.1770. View

19.

Birnboim H, DOLY J . A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979; 7(6):1513-23. PMC: 342324. DOI: 10.1093/nar/7.6.1513. View

20.

Goto K, Takahashi N, Murachi T . Structural studies on stem bromelain. Cyanogen bromide cleavage and amino acid sequence of carboxyl-terminal half of the molecule. Int J Pept Protein Res. 1980; 15(4):335-41. View