Cell Differentiation and Proliferation in the Bone Marrow and Other Organs of 2D2 Mice During Spontaneous Development of EAE Leading to the Production of Abzymes

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Apr 12

PMID 35408594

Authors

Kseniya S Aulova

Andrey E Urusov

Ludmila B Toporkova

Sergey E Sedykh

Juliya A Shevchenko

Valeriy P Tereshchenko

Sergei V Sennikov

Irina A Orlovskaya

Georgy A Nevinsky

Affiliations

Soon will be listed here.

Abstract

The exact cellular and molecular mechanisms of multiple sclerosis and other autoimmune diseases have not been established. Autoimmune pathologies are known to be associated with faults in the immune system and changes in the differentiation profiles of bone marrow stem cells. This study analyzed various characteristics of experimental autoimmune encephalomyelitis (EAE) in 2D2 mice. Differentiation profiles of six hematopoietic stem cells of bone marrow were found to significantly differ in 2D2 male and female mice during the spontaneous development of EAE. In addition, we found various properties of B and T cells, CD4+ and CD8+ lymphocytes in blood and several organs (bone marrow, spleen, thymus, and lymph nodes) of 2D2 male and female mice to be considerably different. These changes in hematopoietic stem cells differentiation profiles and level of lymphocyte proliferation in various organs of 2D2 mice were found to induce the production of IgGs against DNA, myelin basic protein, and myelin oligodendrocyte glycoprotein, increasing the number of autoantibodies hydrolyzing these substrates. We compared the changes of these immunological and biochemical parameters in 2D2 mice with those of mice of two other lines (Th and C57BL/6), also prone to spontaneous development of EAE. Some noticeable and even extreme variations were found in the time-related development of parameters between male and female mice of 2D2, Th, and C57BL/6 lines. Despite some differences, mice of all three lines demonstrated the changes in hematopoietic stem cells profiles, lymphocyte content, and production of catalytic autoantibodies. Given that these changes are harmful to mice, we believe them to cause the development of experimental autoimmune encephalomyelitis.

Citing Articles

Cellular and Immunological Analysis of 2D2/Th Hybrid Mice Prone to Experimental Autoimmune Encephalomyelitis in Comparison with 2D2 and Th Lines.

Aulova K, Urusov A, Chernyak A, Toporkova L, Chicherina G, Buneva V Int J Mol Sci. 2024; 25(18).

PMID: 39337388 PMC: 11432411. DOI: 10.3390/ijms25189900.

Natural IgG against S-Protein and RBD of SARS-CoV-2 Do Not Bind and Hydrolyze DNA and Are Not Autoimmune.

Timofeeva A, Sedykh S, Ermakov E, Matveev A, Odegova E, Sedykh T Int J Mol Sci. 2022; 23(22).

PMID: 36430159 PMC: 9693483. DOI: 10.3390/ijms232213681.

Antibodies-Abzymes with Antioxidant Activities in Two Th and 2D2 Experimental Autoimmune Encephalomyelitis Mice during the Development of EAE Pathology.

Tolmacheva A, Aulova K, Urusov A, Doronin V, Nevinsky G Molecules. 2022; 27(21).

PMID: 36364362 PMC: 9656754. DOI: 10.3390/molecules27217527.

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