Separation, Purification, Structural Characterization, and Anticancer Activity of a Novel Exopolysaccharide from Sp

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Apr 12

PMID 35408470

Authors

Jiaojiao Wang

Pingchuan Yuan

Wenzhi Zhang

Chunyan Liu

Kaoshan Chen

Guodong Wang

Taili Shao

Affiliations

Soon will be listed here.

Abstract

sp. has a wide range of applications in the food fermentation industry. In this study, a novel exopolysaccharide, labeled MSEPS, was separated from sp. fermentation broth through ethanol precipitation and was purified by ion-exchange chromatography, as well as gel filtration column chromatography. MSEPS was composed mostly of mannose, galactose, fucose, arabinose, and glucose with a molar ratio of 0.466:0.169:0.139:0.126:0.015 and had a molecular weight of 7.78 × 10 Da. The analysis of methylation and nuclear magnetic resonance results indicated that MSEPS mainly consisted of a backbone of →3,6)-α-d-Man-(1→3,6)-β-d-Gal-(1→, with substitution at O-3 of →6)-α-d-Man-(1→ and →6)-β-d-Gal-(1→ by terminal α-l-Ara residues. MTT assays showed that MSEPS was nontoxic in normal cells (HK-2 cells) and inhibited the proliferation of carcinoma cells (SGC-7901 cells). Additionally, morphological analysis and flow cytometry experiments indicated that MSEPS promoted SGC-7901 cell death via apoptosis. Therefore, MSEPS from sp. can be developed as a potential antitumor agent.

Citing Articles

First report on isolation of from honeybees, : Characterization and biological activities.

Kalaba M, Sultan M, Elbahnasawy M, El-Didamony S, El Bakary N, Sharaf M Biotechnol Rep (Amst). 2022; 36:e00770.

PMID: 36338578 PMC: 9634281. DOI: 10.1016/j.btre.2022.e00770.

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