Gastrointestinal Protein Hydrolysis Kinetics: Opportunities for Further Infant Formula Improvement

Overview

Journal Nutrients

Date 2022 Apr 12

PMID 35406125

Authors

Evan Abrahamse

Gabriel G M Thomassen

Ingrid B Renes

Peter A Wierenga

Kasper A Hettinga

Affiliations

Soon will be listed here.

Abstract

The postprandial plasma essential amino acid (AA) peak concentrations of infant formula (IF) are higher than those of human milk (HM) in infants. In addition, several HM proteins have been recovered intact in infant stool and appeared digestion resistant in vitro. We, therefore, hypothesized that gastrointestinal protein hydrolysis of IF is faster than HM and leads to accelerated absorbable digestion product release. HM and IF protein hydrolysis kinetics were compared in a two-step semi-dynamic in vitro infant digestion model, and the time course of degree of protein hydrolysis (DH), loss of intact protein, and release of free AA and peptides was evaluated. Gastric DH increase was similar for IF and HM, but the rate of intestinal DH increase was 1.6 times higher for IF than HM. Intact protein loss in IF was higher than HM from 120 min gastric phase until 60 min intestinal phase. Intestinal phase total digestion product (free AA + peptides <5 kDa) concentrations increased ~2.5 times faster in IF than HM. IF gastrointestinal protein hydrolysis and absorbable product release are faster than HM, possibly due to the presence of digestion-resistant proteins in HM. This might present an opportunity to further improve IF bringing it closer to HM.

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References

. Enzymatic hydrolysis of proteins for increased solubility. J Agric Food Chem. 1976; 24(6):1090-3. DOI: 10.1021/jf60208a021. View

Elwakiel M, Boeren S, Wang W, Schols H, Hettinga K . Degradation of Proteins From Colostrum and Mature Milk From Chinese Mothers Using an Infant Digestion Model. Front Nutr. 2020; 7:162. PMC: 7557360. DOI: 10.3389/fnut.2020.00162. View

Blanquet S, Zeijdner E, Beyssac E, Meunier J, Denis S, Havenaar R . A dynamic artificial gastrointestinal system for studying the behavior of orally administered drug dosage forms under various physiological conditions. Pharm Res. 2004; 21(4):585-91. DOI: 10.1023/b:pham.0000022404.70478.4b. View

Armand M, HAMOSH M, Mehta N, Angelus P, Philpott J, Henderson T . Effect of human milk or formula on gastric function and fat digestion in the premature infant. Pediatr Res. 1996; 40(3):429-37. DOI: 10.1203/00006450-199609000-00011. View

Sondheimer J, Clark D, Gervaise E . Continuous gastric pH measurement in young and older healthy preterm infants receiving formula and clear liquid feedings. J Pediatr Gastroenterol Nutr. 1985; 4(3):352-5. DOI: 10.1097/00005176-198506000-00005. View

Luiking Y, Abrahamse E, Ludwig T, Boirie Y, Verlaan S . Protein type and caloric density of protein supplements modulate postprandial amino acid profile through changes in gastrointestinal behaviour: A randomized trial. Clin Nutr. 2015; 35(1):48-58. DOI: 10.1016/j.clnu.2015.02.013. View

Maathuis A, Havenaar R, He T, Bellmann S . Protein Digestion and Quality of Goat and Cow Milk Infant Formula and Human Milk Under Simulated Infant Conditions. J Pediatr Gastroenterol Nutr. 2017; 65(6):661-666. PMC: 5704675. DOI: 10.1097/MPG.0000000000001740. View

Lonnerdal B . Nutritional and physiologic significance of human milk proteins. Am J Clin Nutr. 2003; 77(6):1537S-1543S. DOI: 10.1093/ajcn/77.6.1537S. View

Boirie Y, Dangin M, Gachon P, Vasson M, Maubois J, Beaufrere B . Slow and fast dietary proteins differently modulate postprandial protein accretion. Proc Natl Acad Sci U S A. 1998; 94(26):14930-5. PMC: 25140. DOI: 10.1073/pnas.94.26.14930. View

10.

Bodin A, Framboisier X, Alonso D, Marc I, Kapel R . Size-exclusion HPLC as a sensitive and calibrationless method for complex peptide mixtures quantification. J Chromatogr B Analyt Technol Biomed Life Sci. 2015; 1006:71-79. DOI: 10.1016/j.jchromb.2015.09.035. View

11.

Erickson R, Kim Y . Digestion and absorption of dietary protein. Annu Rev Med. 1990; 41:133-9. DOI: 10.1146/annurev.me.41.020190.001025. View

12.

Smith L, Kaminsky S, DSouza S . Neonatal fat digestion and lingual lipase. Acta Paediatr Scand. 1986; 75(6):913-8. DOI: 10.1111/j.1651-2227.1986.tb10316.x. View

13.

Bourlieu C, Menard O, De La Chevasnerie A, Sams L, Rousseau F, Madec M . The structure of infant formulas impacts their lipolysis, proteolysis and disintegration during in vitro gastric digestion. Food Chem. 2015; 182:224-35. DOI: 10.1016/j.foodchem.2015.03.001. View

14.

Lindberg T, Engberg S, Sjoberg L, Lonnerdal B . In vitro digestion of proteins in human milk fortifiers and in preterm formula. J Pediatr Gastroenterol Nutr. 1998; 27(1):30-6. DOI: 10.1097/00005176-199807000-00006. View

15.

van Lieshout G, Lambers T, Bragt M, Hettinga K . How processing may affect milk protein digestion and overall physiological outcomes: A systematic review. Crit Rev Food Sci Nutr. 2019; 60(14):2422-2445. DOI: 10.1080/10408398.2019.1646703. View

16.

Leeb E, Gotz A, Letzel T, Cheison S, Kulozik U . Influence of denaturation and aggregation of β-lactoglobulin on its tryptic hydrolysis and the release of functional peptides. Food Chem. 2015; 187:545-54. DOI: 10.1016/j.foodchem.2015.04.034. View

17.

Rondini G, Pittaluga F, Sina P, Prigione M . [Lipid digestion and absorption in 1-month-old infants fed with an acidified and non-acidified milk]. Minerva Pediatr. 1972; 24(18):755-61. View

18.

Kuipers B, Gruppen H . Prediction of molar extinction coefficients of proteins and peptides using UV absorption of the constituent amino acids at 214 nm to enable quantitative reverse phase high-performance liquid chromatography-mass spectrometry analysis. J Agric Food Chem. 2007; 55(14):5445-51. DOI: 10.1021/jf070337l. View

19.

van Veen H, Geerts M, van Berkel P, Nuijens J . The role of N-linked glycosylation in the protection of human and bovine lactoferrin against tryptic proteolysis. Eur J Biochem. 2004; 271(4):678-84. DOI: 10.1111/j.1432-1033.2003.03965.x. View

20.

Menard O, Bourlieu C, De Oliveira S, Dellarosa N, Laghi L, Carriere F . A first step towards a consensus static in vitro model for simulating full-term infant digestion. Food Chem. 2017; 240:338-345. DOI: 10.1016/j.foodchem.2017.07.145. View