TMT Labeled Comparative Proteomic Analysis Reveals Spleen Active Immune Responses During Type C Infected Piglet Diarrhea

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2022 Apr 11

PMID 35402105

Authors

Xiaoli Wang

Xiaoyu Huang

Qiaoli Yang

Zunqiang Yan

Pengfei Wang

Xiaoli Gao

Ruirui Luo

Shuangbao Gun

Affiliations

Soon will be listed here.

Abstract

Background: () type C is the principal pathogenic clostridia of swine, frequently causing hemorrhagic diarrhea, even necrotic enteritis in piglets, leading to severe economic loss for swine industr ies worldwide. However, there are no specific and effective prevention measures. Therefore, clarifying the molecular mechanisms of hosts against pathogenesis infection is very important to reduce the incidence of type C infected piglet diarrhea disease.

Methods: We performed an TMT labeling-based quantitative spleen proteomic analysis of the control group (SC), tolerance group (SR) and susceptible group (SS) to identify the differentially expressed proteins (DEPs), and screened potential molecular markers of piglet spleen tissues in response to type C infection.

Results: In this study, a total of 115, 176 and 83 DEPs were identified in SR vs SC, SS vs SC, and SR vs SC, respectively, which may play the important regulatory roles in the process of piglet spleens in response to type C-infected diarrhea diseases. GO enrichment analysis revealed that the DEPs were mostly significantly enriched in acute inflammatory response, defense response, antimicrobial response, transporter activity, cellular metabolic process and so on, and KEGG pathway enrichment analysis showed that the significantly enriched immune related pathways of the PPAR signaling pathway, IL-17 signaling pathway, antigen processing and presentation, which hints at the immune defense process of piglet spleen against infection. This study helps to elucidate the protein expressional pattern of piglet spleen against type C-infected diarrhea disease, which can contribute to the prevention and control for pig diarrhea disease and the further development of diarrhea resistant pig breeding.

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