Evaluation of the Antioxidant and Anticancer Activities of Hydroalcoholic Extracts of Čelak and Benth

Overview

Journal J Toxicol

Publisher Wiley

Specialty Toxicology

Date 2022 Apr 11

PMID 35401741

Authors

Zahra Barmoudeh

Maryam Tajali Ardakani

Amir Hossein Doustimotlagh

Hassan Bardania

Affiliations

Soon will be listed here.

Abstract

Herein, the effects of hydroalcoholic extracts of Celak (TDC) and Benth (SPB) plants on HepG2 cell line were investigated by using different analyses. Cytotoxicity and apoptosis of extracts were investigated by MTT method, AnnV/PI apoptosis assay, and their antioxidant capacity was evaluated by total thiol and glutathione peroxidase (GPX) assay. The results revealed that the SBP extract was more cytotoxic compared with the TDC extract and increased over time (128.49 g/mL vs 107.11 g/mL IC values for 24 and 72 h, respectively). Although, AnnV/PI apoptosis assay showed apoptosis induction for both extracts, but the caspase-3 activity assay revealed that TDC extract significantly increased caspase-3 activity compared with the control and SPB extract. Increasing the activity of GPX by SPB extract revealed that it has high antioxidant capacity. In conclusion, the TDC and SPB with high antioxidant capacity have high cytotoxicity against HepG2 cancer cells and have high capability as a medicinal plant.

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