UHPLC-MS/MS-GNPS Based Phytochemical Investigation of L. And Evaluation of Cytotoxicity Against Human Melanoma and Ovarian Cancer Cells

Overview

Journal Saudi J Biol Sci

Specialty Biology

Date 2022 Apr 8

PMID 35392596

Authors

Zia-Ur-Rehman

Aleksandra Gurgul

Isoo Youn

Amanda Maldonado

Fazli Wahid

Chun-Tao Che

Taous Khan

Affiliations

Soon will be listed here.

Abstract

L. is widely used as a traditional medicine for the management of inflammation and cancer. In the present study, phyto-chemical analysis of was carried out and its cytotoxic potential against human melanoma (MDA-MB-435) and ovarian cancer cells (OVCAR3) was evaluated. Phyto-chemical profile of methanolic extract and its fractions was established employing UHPLC-MS/MS and Global Natural Product Social molecular networking. Cytotoxic activity was evaluated using absorbance assay (CellTiter-Blue® Cell Viability Assay). Overall, 22 compounds were identified in the crude extract and polarity-based fractions of Flavonoids, flavonoid-O-glycosides and phenolic acids were found to be the major classes of phyto-chemicals In addition, the crude extract of and its fractions were found active against the tested cell lines. The highest anti-cancer activity against OVCAR3 cells was exhibited by the -hexane fraction. These results indicated that is rich in flavonoids and might be used for the development of anti-cancer drugs against melanoma and ovarian cancers.

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