Molecular Evaluation of Infection and Its Association with Tumor Necrosis Factor-α Polymorphism in Recurrent Spontaneous Abortions

Overview

Journal Ethiop J Health Sci

Specialty Health Services

Date 2022 Apr 8

PMID 35392343

Authors

Fatemeh Darvishi Kolor

Jalil Vand Yousefi

Reza Ranjbar

Affiliations

Soon will be listed here.

Abstract

Background: The rate of infections in the intensive care units (ICUs) is rising, mainly because of the increasing use of invasive procedures. Several bacterial infections and host genetic backgrounds, including TNFα SNP polymorphisms, play important roles in recurrent spontaneous abortions (RSA). So, this study aimed to evaluate C. trachomatis infection and its relation with TNFα -308 and -238 polymorphism for early detection and treatment of RSA.

Methods: The blood samples were taken from 63 Iranian women with a history of RSA and 59 ethnically matched healthy controls with at least two successful pregnancies and no history of abortion. Then, DNA was extracted from all samples and detection of C. trachomatis and TNFα -308 and -238 polymorphism was determined by multiplex amplification-refractory mutation system (ARMS)-PCR. Finally, the statistical analysis to detection C. trachomatis and host genetic roles in RSA were analyzed by Epi Info software by X test.

Results: C. trachomatis was detected in 22 and 3% of the RSA and the control group, respectively. Moreover, in comparison with healthy controls, C. trachomatis infection was closely correlated with TNFα -308 genotypes, whereas no significant association was observed between TNFα -238G/A and RSA. In addition, statistical analysis of TNFα -308 genotypes showed that the frequency of genotype AA was higher in patients with C. trachomatis infections than healthy individuals and the difference was statistically significant.

Conclusions: This study demonstrated that molecular analysis of TNF -308 genotypes is important in early detection and treatment of RSA with C. trachomatis infection.

Citing Articles

Prevalence of and in Infertile Couples and the Effect on Semen Parameters.

Ahmadi K, Moosavian M, Mardaneh J, Pouresmaeil O, Afzali M Ethiop J Health Sci. 2023; 33(1):133-142.

PMID: 36890937 PMC: 9987280. DOI: 10.4314/ejhs.v33i1.17.

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