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Role of MiR-181b/Notch1 Axis in Circ_TNPO1 Promotion of Proliferation and Migration of Atherosclerotic Vascular Smooth Muscle Cells

Overview

Overview

Journal J Healthc Eng

Specialty Biomedical Engineering

Date 2022 Apr 7

PMID 35388333

Authors

Authors

Mingxiang Chen

Fuping Li

Qilong Jiang

Weimin Zhang

Zhiping Li

Wenshuai Tang

Affiliations

Affiliations

Soon will be listed here.

Abstract

Background: The role and expression level change in circ_TNPO1 (hsa_circ_0072951) in atherosclerosis (AS) and VSMC dysfunction remain unknown. In this study, we try to explore the effects of circ_TNPO1 on oxidized low-density lipoprotein (ox-LDL)-induced human vascular smooth muscle cell (VSMC) excessive proliferation and migration, and the potential molecular mechanism.

Methods: Quantitative real-time polymerase chain reaction (RT-qPCR) and western blot experiment were used to detect the serum samples from AS patients and healthy controls. CCK-8, Transwell, and the dual-luciferase reporter gene assay were used to detect the cell biology.

Results: In human AS serum and ox-LDL-induced VSMCs, circ_TNPO1 was increased, whereas miR-181b was decreased. Silencing circ_TNPO1 inhibited proliferation and migration activity and reduced protein expression of PCNA, Ki-67, MMP2, and E-cadherin and promoted N-cadherin protein expression in ox-LDL induced VSMCs. Remarkably, miR-181b knockdown or Notch1 overexpression could efficiently offset the proliferation and migration inhibiting effect of circ_TNPO1 knockdown in ox-LDL-induced VSMCs. Furthermore, a molecular mechanism study pointed out that circ_TNPO1 and Notch1 are direct-acting targets of miR-181b.

Conclusions: In conclusion, our study indicated that circ_TNPO1 promotes the proliferation and migration progression of VSMCs in atherosclerosis through the miR-181b/Notch1 axis.

Citing Articles

Circular RNAs in vascular diseases.

Liu Q, Wang Y, Zhang T, Fang J, Meng S Front Cardiovasc Med. 2023; 10:1247434.

PMID: 37840954 PMC: 10570532. DOI: 10.3389/fcvm.2023.1247434.

Retracted: Role of miR-181b/Notch1 Axis in circ_TNPO1 Promotion of Proliferation and Migration of Atherosclerotic Vascular Smooth Muscle Cells.

Healthcare Engineering J J Healthc Eng. 2023; 2023:9835702.

PMID: 37799659 PMC: 10550460. DOI: 10.1155/2023/9835702.

Circular RNAs Variously Participate in Coronary Atherogenesis.

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PMID: 37623241 PMC: 10453518. DOI: 10.3390/cimb45080422.

Circular RNA as Therapeutic Targets in Atherosclerosis: Are We Running in Circles?.

Triska J, Mathew C, Zhao Y, Chen Y, Birnbaum Y J Clin Med. 2023; 12(13).

PMID: 37445481 PMC: 10342353. DOI: 10.3390/jcm12134446.

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