Isolation, Characterization and Evaluation of Specific Bacteriophages Targeting Extensive Drug Resistance Strains of Isolated from Septic Burn Wounds

Overview

Journal Infect Chemother

Specialty Infectious Diseases

Date 2022 Apr 6

PMID 35384426

Authors

Seyed Mahmoud Amin Marashi

Farhad Nikkhahi

Dariush Hamedi

Gholamhassan Shahbazi

Affiliations

Soon will be listed here.

Abstract

Background: Antibiotic resistant bacteria and various infections caused by them especially extensive drug resistance (XDR) strains and worrying statistics of mortality due to these strains and also the lack of a clear vision for development and production of new effective antibiotics have made the necessity of using alternative therapies more apparent.

Materials And Methods: In this study, specific phages affecting the XDR strain were extracted from hospital wastewater and their laboratory characteristics along with lysis effect on 40 XDR strains of were investigated.

Results: The results indicated that three isolated phages (PaB1, PaBa2 and PaBa3) belonged to the and families and were specific to strains. More than 98% of phages absorbed their host in less than 10 minutes (Adsorption time <10 min) and completed their lytic cycle after 40 minutes (latent time = 40 min). Burst size of PaBa1, PaBa2 and PaBa3 was 240, 250 and 220 pfu/cell, respectively. PaBa1 lysed 62.5% of the XDR strains with the highest efficiency. The three Phage cocktail was effective against 67.5% of the studied strains.

Conclusion: The results of this study indicate the significant potential of these phages for therapeutic use and prophylaxis of infections caused by this bacterium.

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