Tandemly Duplicated Upstream Control Sequences Mediate Copper-induced Transcription of the Saccharomyces Cerevisiae Copper-metallothionein Gene

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1986 Apr 1

PMID 3537699

Citations 54

Authors

D J Thiele

D H Hamer

Affiliations

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Abstract

Transcription of the Saccharomyces cerevisiae copper-metallothionein gene, CUP1, inducible by copper. By analyzing deletion and fusion mutants in the CUP1 5'-flanking region, we identified two closely related, tandemly arranged copper regulatory elements. A synthetic version of one of these elements conferred efficient copper induction on a heterologous promoter when present in two tandem copies.

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References

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Winge D, Nielson K, Gray W, Hamer D . Yeast metallothionein. Sequence and metal-binding properties. J Biol Chem. 1985; 260(27):14464-70. View

Fogel S, Welch J . Tandem gene amplification mediates copper resistance in yeast. Proc Natl Acad Sci U S A. 1982; 79(17):5342-6. PMC: 346892. DOI: 10.1073/pnas.79.17.5342. View

Donahue T, Daves R, Lucchini G, Fink G . A short nucleotide sequence required for regulation of HIS4 by the general control system of yeast. Cell. 1983; 32(1):89-98. DOI: 10.1016/0092-8674(83)90499-3. View

Guarente L, Mason T . Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site. Cell. 1983; 32(4):1279-86. DOI: 10.1016/0092-8674(83)90309-4. View

Messing J . New M13 vectors for cloning. Methods Enzymol. 1983; 101:20-78. DOI: 10.1016/0076-6879(83)01005-8. View

Welch J, Fogel S, Cathala G, Karin M . Industrial yeasts display tandem gene iteration at the CUP1 region. Mol Cell Biol. 1983; 3(8):1353-61. PMC: 369981. DOI: 10.1128/mcb.3.8.1353-1361.1983. View

Guarente L, Lalonde B, Gifford P, Alani E . Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiae. Cell. 1984; 36(2):503-11. DOI: 10.1016/0092-8674(84)90243-5. View

Rymond B, Zitomer R, Schumperli D, Rosenberg M . The expression in yeast of the Escherichia coli galK gene on CYC1::galK fusion plasmids. Gene. 1983; 25(2-3):249-62. DOI: 10.1016/0378-1119(83)90229-9. View

10.

Karin M, Najarian R, Haslinger A, Valenzuela P, Welch J, Fogel S . Primary structure and transcription of an amplified genetic locus: the CUP1 locus of yeast. Proc Natl Acad Sci U S A. 1984; 81(2):337-41. PMC: 344671. DOI: 10.1073/pnas.81.2.337. View

11.

Hannig E, Thiele D, Leibowitz M . Saccharomyces cerevisiae killer virus transcripts contain template-coded polyadenylate tracts. Mol Cell Biol. 1984; 4(1):101-9. PMC: 368663. DOI: 10.1128/mcb.4.1.101-109.1984. View

12.

Karin M, Haslinger A, Holtgreve H, Richards R, Krauter P, Westphal H . Characterization of DNA sequences through which cadmium and glucocorticoid hormones induce human metallothionein-IIA gene. Nature. 1984; 308(5959):513-9. DOI: 10.1038/308513a0. View

13.

Butt T, Sternberg E, Gorman J, Clark P, Hamer D, Rosenberg M . Copper metallothionein of yeast, structure of the gene, and regulation of expression. Proc Natl Acad Sci U S A. 1984; 81(11):3332-6. PMC: 345501. DOI: 10.1073/pnas.81.11.3332. View

14.

Weir J, Moss B . Regulation of expression and nucleotide sequence of a late vaccinia virus gene. J Virol. 1984; 51(3):662-9. PMC: 255823. DOI: 10.1128/JVI.51.3.662-669.1984. View

15.

Stuart G, Searle P, Chen H, Brinster R, Palmiter R . A 12-base-pair DNA motif that is repeated several times in metallothionein gene promoters confers metal regulation to a heterologous gene. Proc Natl Acad Sci U S A. 1984; 81(23):7318-22. PMC: 392137. DOI: 10.1073/pnas.81.23.7318. View

16.

Carter A, Felber B, Walling M, Jubier M, Schmidt C, Hamer D . Duplicated heavy metal control sequences of the mouse metallothionein-I gene. Proc Natl Acad Sci U S A. 1984; 81(23):7392-6. PMC: 392152. DOI: 10.1073/pnas.81.23.7392. View

17.

Guarente L, Hoar E . Upstream activation sites of the CYC1 gene of Saccharomyces cerevisiae are active when inverted but not when placed downstream of the "TATA box". Proc Natl Acad Sci U S A. 1984; 81(24):7860-4. PMC: 392252. DOI: 10.1073/pnas.81.24.7860. View

18.

Struhl K . Genetic properties and chromatin structure of the yeast gal regulatory element: an enhancer-like sequence. Proc Natl Acad Sci U S A. 1984; 81(24):7865-9. PMC: 392253. DOI: 10.1073/pnas.81.24.7865. View

19.

Hinnebusch A, Lucchini G, Fink G . A synthetic HIS4 regulatory element confers general amino acid control on the cytochrome c gene (CYC1) of yeast. Proc Natl Acad Sci U S A. 1985; 82(2):498-502. PMC: 397066. DOI: 10.1073/pnas.82.2.498. View

20.

Giniger E, Varnum S, Ptashne M . Specific DNA binding of GAL4, a positive regulatory protein of yeast. Cell. 1985; 40(4):767-74. DOI: 10.1016/0092-8674(85)90336-8. View