Structural Basis for Different Types of Hetero-tetrameric Light-harvesting Complexes in a Diatom PSII-FCPII Supercomplex

Overview

Journal Nat Commun

Specialty Biology

Date 2022 Apr 2

PMID 35365610

Authors

Ryo Nagao

Koji Kato

Minoru Kumazawa

Kentaro Ifuku

Makio Yokono

Takehiro Suzuki

Naoshi Dohmae

Fusamichi Akita

Seiji Akimoto

Naoyuki Miyazaki

Jian-Ren Shen

Affiliations

Soon will be listed here.

Abstract

Fucoxanthin chlorophyll (Chl) a/c-binding proteins (FCPs) function as light harvesters in diatoms. The structure of a diatom photosystem II-FCPII (PSII-FCPII) supercomplex have been solved by cryo-electron microscopy (cryo-EM) previously; however, the FCPII subunits that constitute the FCPII tetramers and monomers are not identified individually due to their low resolutions. Here, we report a 2.5 Å resolution structure of the PSII-FCPII supercomplex using cryo-EM. Two types of tetrameric FCPs, S-tetramer, and M-tetramer, are identified as different types of hetero-tetrameric complexes. In addition, three FCP monomers, m1, m2, and m3, are assigned to different gene products of FCP. The present structure also identifies the positions of most Chls c and diadinoxanthins, which form a complicated pigment network. Excitation-energy transfer from FCPII to PSII is revealed by time-resolved fluorescence spectroscopy. These structural and spectroscopic findings provide insights into an assembly model of FCPII and its excitation-energy transfer and quenching processes.

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