A Cumulative Effect by Multiple-Gene Knockout Strategy Leads to a Significant Increase in the Production of Sophorolipids in CGMCC 1576

Overview

Journal Front Bioeng Biotechnol

Date 2022 Mar 31

PMID 35356780

Authors

Jun Liu

Xinyu Zhang

Guodong Liu

Guoqin Zhao

Xiaoran Fang

Xin Song

Affiliations

Soon will be listed here.

Abstract

Sophorolipids (SLs), an important biosurfactant produced by , were one of the most potential substitutes for chemical surfactants. Few reports on the transcriptional regulation of SLs synthesis and the engineered strains with high-yield SLs were available. In this study, a Rim9-like protein (Rlp) and three transcription factors (, , ) were mined and analyzed, and a progressive enhancement of SLs production was achieved through cumulative knockouts of three genes. The sophorolipid production of reached 97.44 g/L, increased by 50.51% than that of the wild-type strain. Compared with the wild-type strain, the flow of glucose to SLs synthesis pathways was increased, and the synthesis of branched-chain amino acids was reduced in . The amount of UDP-glucose, the substrate for two glycosyltransferases, also increased, and the expression level of the key genes and for SLs synthesis increased by 2.2 times, respectively. The multiple-gene knockout strategy was proved to be highly effective to construct the engineered strain with high-yield SLs production, and this strain was a superior strain for industrial fermentation of SLs and reduced SLs production costs.

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