Blume Induces Apoptosis Against Acute Myeloid Leukemia Cells Regulation of the MiR-216b/c-Jun

Overview

Journal Front Oncol

Specialty Oncology

Date 2022 Mar 31

PMID 35356209

Authors

Moon Nyeo Park

Hee Won Jeon

Md Ataur Rahman

Se Sun Park

Se Yun Jeong

Ki Hyun Kim

Sung-Hoon Kim

Woojin Kim

Bonglee Kim

Affiliations

Soon will be listed here.

Abstract

Blume (DD), also called dragon's blood, has been used as a traditional Korean medicine, especially for relieving pain caused by wound infection. Recently, it has been described that DD has antibacterial and analgesic effects. In this study, the underlying anticancer effect of DD associated with apoptosis was investigated in acute myeloid leukemia cell lines U937 and THP-1. DD exhibited cytotoxic effects and induced apoptosis in U937 and THP-1 cells. Moreover, DD treatment significantly reduced mitochondrial membrane potential (ΔΨ). The protein expression of cleaved poly(ADP-ribose) polymerase, cleaved caspase-3, p-H2A.X, CCAAT/enhancer-binding protein (CHOP), and activating transcription factor 4 was upregulated by DD treatment. Consistently, DD-treated cells had increased reactive oxygen species (ROS) level in a concentration-dependent manner miR-216b activation in association with c-Jun inhibition. N-acetyl-L-cysteine pretreatment reversed the cytotoxic effect of DD treatment as well as prevented ROS accumulation. Collectively, the results of this study suggest that the anticancer effect of DD in AML was mediated by CHOP-dependent apoptosis along with ROS accumulation and included upregulation of miR-216b followed by a decrease in c-Jun.

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