Circ Promotes Myoblast Proliferation and Inhibits Differentiation by Sponging to Release

Overview

Journal Epigenetics

Specialty Genetics

Date 2022 Mar 29

PMID 35348434

Authors

Jian Wang

Yifan Wen

Jiawei Xu

Binglin Yue

Jialin Zhong

Li Zheng

Chuzhao Lei

Hong Chen

Yongzhen Huang

Affiliations

Soon will be listed here.

Abstract

Muscle development is a complex process that was regulated by many factors, among which non-coding RNAs (ncRNAs) play a vital role in regulating multiple life activities of muscle cells. Circular RNA (circRNA), a type of non-coding RNA with closed-loop structure, has been reported to affect multiple life processes. However, the roles of circRNAs on muscle development have not been fully elucidated. The present study aimed to determine whether and how circ affects muscle development. Our study revealed that circ promoted myoblast proliferation and inhibited differentiation. Besides, c was proved as a downstream target of circ using dual-luciferase reporter assay and RNA-binding protein immunoprecipitation (RIP) assay. Also, potassium inwardly rectifying channel subfamily J member 12 () was identified as a novel target of via dual-luciferase reporter assay, quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR), and western blot. Circ and were both proved to regulate cell cycle on muscle regeneration after injury . In conclusion, we demonstrated that circ sponged , releasing to regulate myoblast proliferation and differentiation and regulating cell cycle during muscle regeneration after injury .

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