Ultrabright Silicon Nanoparticle Fluorescence Probe for Sensitive Detection of Cholesterol in Human Serum

Overview

Journal Anal Bioanal Chem

Specialty Chemistry

Date 2022 Mar 29

PMID 35347354

Authors

Xiwen Ye

Yanxiao Jiang

Xiaowei Mu

Ying Sun

Pinyi Ma

Ping Ren

Daqian Song

Affiliations

Soon will be listed here.

Abstract

A highly sensitive fluorescence-based assay for cholesterol detection was developed using water-dispersible green-emitting silicon nanoparticles (SiNPs) as a fluorescence indicator and enzyme-catalyzed oxidation product PPDox (Bandrowski's base) as a quencher. The SiNPs were facilely synthesized via a simple, one-step hydrothermal treatment using 3-[2-(2-aminoethylamino)ethylamino]propyl-trimethoxysilane (AEEA) as the silicon source, which has ultrahigh quantum yield and low phototoxicity. Under the catalysis of cholesterol oxidase (ChOx), hydrogen peroxide (HO) was generated as a result of cholesterol oxidation. Utilizing p-phenylenediamine (PPD) as the substrate for horseradish peroxidase (HRP) in the presence of HO led to the production of PPDox. Based upon the inner filter effect (IFE), the established ultrasensitive fluorescent assay could accurately measure cholesterol. The limit of detection (LOD) of the assay was 0.018 μM with a linear range of 0.025-10 μM. The results for the detection of real serum samples by the proposed assay were comparable to those by a commercial reagent kit, demonstrating that our proposed strategy has high application potential in disease diagnosis and other related biological studies.

Citing Articles

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